Virus-mimetic nanovesicles as a versatile antigen-delivery system

被引：120

作者：

Zhang, Pengfei ^{[1
,2
]}

Chen, Yixin ^{[3
,4
,5
]}

Zeng, Yun ^{[1
,2
,6
]}

Shen, Chenguang ^{[1
,2
,3
,4
]}

Li, Rui ^{[1
,2
,3
,4
]}

Guo, Zhide ^{[1
,2
]}

Li, Shaowei ^{[1
,2
,3
,4
,5
]}

Zheng, Qingbing ^{[1
,2
,3
,4
]}

Chu, Chengchao ^{[1
,2
]}

Wang, Zhantong ^{[1
,2
,7
]}

Zheng, Zizheng ^{[1
,2
,3
,4
]}

Tian, Rui ^{[1
,2
]}

Ge, Shengxiang ^{[1
,2
,3
,4
]}

Zhang, Xianzhong ^{[1
,2
]}

Xia, Ning-Shao ^{[1
,2
,3
,4
,5
]}

Liu, Gang ^{[1
,2
,5
,8
]}

Chen, Xiaoyuan ^{[7
]}

机构：

[1] Xiamen Univ, Sch Publ Hlth, State Key Lab Mol Vaccinol & Mol Diagnost, Xiamen 361102, Peoples R China

[2] Xiamen Univ, Sch Publ Hlth, Ctr Mol Imaging & Translat Med, Xiamen 361102, Peoples R China

[3] Xiamen Univ, Sch Life Sci, Natl Inst Diagnost & Vaccine Dev Infect Dis, Xiamen 361102, Peoples R China

[4] Xiamen Univ, Sch Publ Hlth, Xiamen 361102, Peoples R China

[5] Xiamen Univ, Sch Life Sci, Innovat Ctr Cell Biol, State Key Lab Cellular Stress Biol, Xiamen 361102, Peoples R China

[6] Sichuan Univ, West China Sch Preclin & Forens Med, Dept Pharmacol, Chengdu 610041, Peoples R China

[7] Natl Inst Biomed Imaging & Bioengn, Lab Mol Imaging & Nanomed, NIH, Bethesda, MD 20892 USA

[8] Xiamen Univ, Coll Chem & Chem Engn, MOE Key Lab Spectrochem Anal & Instrumentat, Xiamen 361005, Peoples R China

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2015年 / 112卷 / 45期

基金：

中国国家自然科学基金; 美国国家卫生研究院;

关键词：

virus-mimetic vesicle; vaccine; nanobiotechnology; antigen delivery system; cell membrane; DRUG-DELIVERY; BILE-SALTS; VACCINES; HEMAGGLUTININ; CELLS; NANOPARTICLES; VACCINATION; INFECTION; TRANSPORT; CARRIERS;

D O I：

10.1073/pnas.1505799112

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

It is a critically important challenge to rapidly design effective vaccines to reduce the morbidity and mortality of unexpected pandemics. Inspired from the way that most enveloped viruses hijack a host cell membrane and subsequently release by a budding process that requires cell membrane scission, we genetically engineered viral antigen to harbor into cell membrane, then form uniform spherical virus-mimetic nanovesicles (VMVs) that resemble natural virus in size, shape, and specific immunogenicity with the help of surfactants. Incubation of major cell membrane vesicles with surfactants generates a large amount of nano-sized uniform VMVs displaying the native conformational epitopes. With the diverse display of epitopes and viral envelope glycoproteins that can be functionally anchored onto VMVs, we demonstrate VMVs to be straightforward, robust and tunable nanobiotechnology platforms for fabricating antigen delivery systems against a wide range of enveloped viruses.

引用

页码：E6129 / E6138

页数：10

共 32 条

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