Fast hyperspectral phase and amplitude imaging in scattering tissue

被引：4

作者：

Ba, Gong ^{[1
]}

Tsang, Jean-Marc ^{[1
]}

Mertz, Jerome ^{[1
]}

机构：

[1] Boston Univ, Biomed Engn Dept, 44 Cummington Mall, Boston, MA 02215 USA

来源：

OPTICS LETTERS | 2018年 / 43卷 / 09期

基金：

美国国家卫生研究院;

关键词：

THICK TISSUE; MICROSCOPY; SPECTROMETER; SPECTROSCOPY; CONTRAST; ILLUMINATION;

D O I：

10.1364/OL.43.002058

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Hyperspectral imaging in scattering tissue generally suffers from low light collection efficiency. In this Letter, we propose a microscope based on Fourier transform spectroscopy and oblique back-illumination microscopy that provides hyperspectral phase and amplitude images of thick, scattering samples with high throughput. Images can be acquired at >0.1 Hz rates with spectral resolution better than 200 cm(-1), over a wide spectral range of 450-1700 nm. Proof-of-principle demonstrations are presented with chorioallantoic membrane of a chick embryo, illustrating the possibility of high-resolution hemodynamics imaging in thick tissue, based on transmission contrast. (C) 2018 Optical Society of America

引用

页码：2058 / 2061

页数：4

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