Interaction of ribosomal protein L22 with casein kinase 2α: A novel mechanism for understanding the biology of non-small cell lung cancer

被引:15
作者
Yang, Mingxia [1 ]
Sun, Haibo [2 ]
He, Ji [4 ]
Wang, Hong [3 ]
Yu, Xiaowei [1 ]
Ma, Lei [2 ]
Zhu, Changliang [2 ]
机构
[1] Nanjing Med Univ, Affiliated Changzhou Peoples Hosp 2, Dept Resp Med, Changzhou 213000, Jiangsu, Peoples R China
[2] Nanjing Med Univ, Dept Pathogen Biol, Nanjing 210029, Jiangsu, Peoples R China
[3] Nanjing Med Univ, Affiliated Hosp 1, Dept Resp Med, Nanjing 210029, Jiangsu, Peoples R China
[4] Xiamen Entry Exit Inspect & Quarantine Bur, State Key Lab Monitoring & Detect Med Vectors, Xiamen 361012, Fujian, Peoples R China
基金
中国国家自然科学基金;
关键词
non-small cell lung cancer; ribosomal protein L22; TAP; protein-protein interaction; casein kinase 2 alpha; phosphorylation; DOWN-REGULATION; CK2; ACTIVATION; TUMORS; GENE; RNA; RIBOSOMAL-PROTEIN-L22; IDENTIFICATION; SPECIFICITY; EXPRESSION;
D O I
10.3892/or.2014.3187
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Dysfunction of ribosomal proteins (RPs) may play an important role in molecular tumorigenesis, such as lung cancer, acting in extraribosomal functions. Many protein-protein interaction studies and genetic screens have confirmed the extraribosomal capacity of RPs. As reported, ribosomal protein L22 (RPL22) dysfunction could increase cancer risk. In the present study, we examined RPL22-protein complexes in lung cancer cells. Tandem affinity purification (TAP) was used to screen the RPL22-protein complexes, and GST pull-down experiments and confocal microscopy were used to assess the protein-protein interaction. The experiment of kinase assay was used to study the function of the RPL22-protein complexes. The results showed that several differentially expressed proteins were isolated and identified by LC-MS/MS, which revealed that one of the protein complexes included casein kinase 2 alpha (CK2 alpha). RPL22 and CK2 alpha interact in vitro. RPL22 also inhibited CK2 alpha substrate phosphorylation in vitro. This is the first report of the RPL22-CK2 alpha relationship in lung cancer. Dysregulated CK2 may impact cell proliferation and apoptosis, key features of cancer cell biology. Our results indicate that RPL22 may be a candidate anticancer agent due to its CK2 alpha-binding and -inhibitory functions in human lung cancer.
引用
收藏
页码:139 / 144
页数:6
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