New CRISPR-Cas9 vectors for genetic modifications of Bacillus species

被引：29

作者：

Toymentseva, Anna A. ^{[1
]}

Altenbuchner, Josef ^{[1
]}

机构：

[1] Univ Stuttgart, Inst Ind Genet, Allmandring 31, D-70569 Stuttgart, Germany

来源：

FEMS MICROBIOLOGY LETTERS | 2019年 / 366卷 / 01期

关键词：

CRISPR-Cas9; plasmids; transformation; mating/conjugation; NHEJ; Bacillus subtilis; TETRACYCLINE RESISTANCE DETERMINANT; SEQUENCE-SPECIFIC CONTROL; XYLOSE UTILIZATION; EXPRESSION SYSTEM; ESCHERICHIA-COLI; DNA; DELETION; GENOME; CLONING; TRANSFORMATION;

D O I：

10.1093/femsle/fny284

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Genetic manipulation is a fundamental procedure for the study of gene and operon functions and new characteristics acquisition. Modern CRISPR-Cas technology allows genome editing more precisely and increases the efficiency of transferring mutations in a variety of hard to manipulate organisms. Here, we describe new CRISPR-Cas vectors for genetic modifications in bacillary species. Our plasmids are single CRISPR-Cas plasmids comprising all components for genome editing and should be functional in a broad host range. They are highly efficient (up to 97%) and precise. The employment and delivery of these plasmids to bacillary strains can be easily achieved by conjugation from Escherichia coli. During our research we also demonstrated the absence of compatibility between CRISPR-Cas system and non-homologous end joining in Bacillus subtilis.

引用

页数：8

共 51 条

[1] Editing of the Bacillus subtilis Genome by the CRISPR-Cas9 System [J].

Altenbuchner, Josef .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2016, 82 (17) :5421-5427

[2] Double-strand break repair in bacteria: a view from Bacillus subtilis [J].

Ayora, Silvia ;

Carrasco, Begona ;

Cardenas, Paula P. ;

Cesar, Carolina E. ;

Canas, Cristina ;

Yadav, Tribhuwan ;

Marchisone, Chiara ;

Alonso, Juan C. .

FEMS MICROBIOLOGY REVIEWS, 2011, 35 (06) :1055-1081

[3] Single plasmid systems for inducible dual protein expression and for CRISPR-Cas9/CRISPRi gene regulation in lactic acid bacterium Lactococcus lactis [J].

Berlec, Ales ;

Skrlec, Katja ;

Kocjan, Janja ;

Olenic, Maria ;

Strukelj, Borut .

SCIENTIFIC REPORTS, 2018, 8

[4] Inhibition of NHEJ repair by type II-A CRISPR-Cas systems in bacteria [J].

Bernheim, Aude ;

Calvo-Villamanan, Alicia ;

Basier, Clovis ;

Cui, Lun ;

Rocha, Eduardo P. C. ;

Touchon, Marie ;

Bikard, David .

NATURE COMMUNICATIONS, 2017, 8

[5] CRISPR-Cas Systems in Bacteria and Archaea: Versatile Small RNAs for Adaptive Defense and Regulation [J].

Bhaya, Devaki ;

Davison, Michelle ;

Barrangou, Rodolphe .

ANNUAL REVIEW OF GENETICS, VOL 45, 2011, 45 :273-297

[6] CHROMOSOMAL INTEGRATION OF PLASMID DNA BY HOMOLOGOUS RECOMBINATION IN ENTEROCOCCUS-FAECALIS AND LACTOCOCCUS-LACTIS SUBSP LACTIS HOSTS HARBORING TN919 [J].

CASEY, J ;

DALY, C ;

FITZGERALD, GF .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1991, 57 (09) :2677-2682

[7] ONE-STEP PREPARATION OF COMPETENT ESCHERICHIA-COLI - TRANSFORMATION AND STORAGE OF BACTERIAL-CELLS IN THE SAME SOLUTION [J].

CHUNG, CT ;

NIEMELA, SL ;

MILLER, RH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (07) :2172-2175

[8] All seven comG open reading frames are required for DNA binding during transformation of competent Bacillus subtilis [J].

Chung, YS ;

Dubnau, D .

JOURNAL OF BACTERIOLOGY, 1998, 180 (01) :41-45

[9] High-Efficiency Multiplex Genome Editing of Streptomyces Species Using an Engineered CRISPR/Cas System [J].

Cobb, Ryan E. ;

Wang, Yajie ;

Zhao, Huimin .

ACS SYNTHETIC BIOLOGY, 2015, 4 (06) :723-728

[10] Multiplex Genome Engineering Using CRISPR/Cas Systems [J].

Cong, Le ;

Ran, F. Ann ;

Cox, David ;

Lin, Shuailiang ;

Barretto, Robert ;

Habib, Naomi ;

Hsu, Patrick D. ;

Wu, Xuebing ;

Jiang, Wenyan ;

Marraffini, Luciano A. ;

Zhang, Feng .

SCIENCE, 2013, 339 (6121) :819-823

← 1 2 3 4 5 6 →