Level of sialic acid residues in platelet proteins in diabetes, aging, and Hodgkin's lymphoma: a potential role of free radicals in desialylation

The present study evaluated the levels of sialic acid and carbonylation in platelet proteins from diabetes, old age, and Hodgkin's lymphoma cases and explored the role of free radicals in desialylation. Sialic acid and carbonylation were estimated in platelet suspension obtained from 10 healthy adult control subjects, 20 elderly healthy persons, 20 cases with diabetes, and 10 with Hodgkin's lymphoma. The level of sialic acid residues in platelets was found to be significantly lower and the carbonylation of proteins was higher in old age, diabetes, and lymphoma cases in comparison to controls. The level of carbonylation correlated with desialylation in these cases. In vitro treatment of platelets with free radicals was found to cause desialylation and to increase the carbonyl content. Addition of 2-mercaptoethanol, an antioxidant, significantly attenuated the above effects. The study indicates the possible role of free radicals in desialylation of platelet proteins in the above clinical conditions. © 2002 Elsevier Science (USA). All rights reserved.