SCD1 methylation in subcutaneous adipose tissue associated with menopausal age

被引：3
作者：
Lu, S. [1 ]
Xu, F. [2 ]
Hu, W. [1 ]
Niu, Z. [3 ]
Cai, H. [3 ]
Chen, Y. [4 ]
Tu, Q. [4 ]
Zhang, Y. [1 ]
Chen, W. [5 ]
Liu, W. [1 ]
Tang, S. [1 ]
Zhang, Z. [1 ]
机构：
[1] Hangzhou Matern & Child Hlth Care Hosp, Dept Obstet & Gynecol, Hangzhou Womens Hosp, 369 Kunpeng Rd, Hangzhou 310008, Zhejiang, Peoples R China
[2] Hangzhou Third Peoples Hosp, Dept Gastroenterol, Hangzhou, Zhejiang, Peoples R China
[3] Nanjing Med Univ, Dept Obstet & Gynecol, Affiliated Hangzhou Hosp, Hangzhou, Zhejiang, Peoples R China
[4] Nanjing Med Univ, Lab Gene Diag, Affiliated Hangzhou Hosp, Hangzhou, Zhejiang, Peoples R China
[5] Second Peoples Hosp Tonglu, Dept Obstet & Gynecol, Hangzhou, Zhejiang, Peoples R China
来源：
CLIMACTERIC | 2019年 / 22卷 / 04期
关键词：
DNA methylation; stearoyl-coenzyme A desaturase 1; menopausal age; estrogen replacement therapy; DNA methyltransferases; mRNA; HORMONE REPLACEMENT THERAPY; ELEMENT DNA METHYLATION; ENERGY-EXPENDITURE; GENE-EXPRESSION; PATTERNS; INSIGHTS; CANCER; SENSITIVITY; METABOLISM; ESTROGEN;
D O I：
10.1080/13697137.2019.1571028
中图分类号：
R71 [妇产科学];
学科分类号：
100211 ;
摘要：
Objective: This study was designed to investigate associations between menopausal age and the DNA methylation levels of stearoyl-coenzyme A desaturase 1 (SCD1, selected by the Illumina Human Methylation 450K Bead Chip) and explore the changes in mRNA levels of SCD1 and DNA methyltransferases (DNMTs) in response to estrogen replacement therapy (ERT).Methods: In the human experiment, we performed subcutaneous adipose tissue DNA extraction on 85 menopausal women. Methylation of SCD1 was measured by MethyLight polymerase chain reaction. In the rat experiment, we established models of menopause (ovariectomy group) and ERT (ovariectomy + 17-estradiol group). The mRNA levels of SCD1, DNMT1, DNMT3A, and DNMT3B were determined.Results: The results showed that DNA methylation of SCD1 was inversely correlated with menopausal age (r=0.370, P<0.001). In the rat study, the mRNA levels of SCD1 decreased (P<0.001) and those of DNMT3A (P<0.001) and DNMT3B increased (P<0.001) after ERT.Conclusion: Methylation levels of SCD1 were significantly associated with menopausal age. DNMT3A and DNMT3B may be involved in the methylation of SCD1.
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收藏
页码：395 / 402
页数：8
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