Pressurized planar electrochromatography as a supporting tool for qualitative toxicological chemical analysis with thin-layer chromatography and UV-Vis spectrometry

被引：2
作者：
Dzido, Tadeusz H. [1 ]
Kopcial, Ewelina [1 ,2 ]
Szponar, Jaroslaw [3 ,4 ]
Krajewska, Anna [3 ,4 ]
Aftyka, Lidia [5 ]
Bakiera, Jaroslaw [5 ]
Polak, Beata [1 ]
Klimek-Turek, Anna [1 ]
机构：
[1] Med Univ Lublin, Med Analyt Div, Fac Pharm, Dept Phys Chem, Chodzki Str 4a, PL-20093 Lublin, Poland
[2] Med Univ Lublin, Med Analyt Div, Fac Pharm, Dept Bromatol, Lublin, Poland
[3] Med Univ Lublin, Toxicol Clin, Lublin, Poland
[4] Stefan Wyszynski Reg Specialist Hosp, Clin Dept Toxicol & Cardiol, Lublin, Poland
[5] Stefan Wyszynski Reg Specialist Hosp, Dept Lab Diagnost Coagulat & Microbiol, Lublin, Poland
来源：
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2017年 / 40卷 / 5-6期
关键词：
Combined fit factor; drugs; HPTLC; individual fit factor; pressurized planar electrochromatography; qualitative toxicological chemical analysis; thin-layer chromatography; CLOSED-SYSTEM; FORENSIC TOXICOLOGY; SAMPLE APPLICATION; MASS-SPECTROMETRY; LIBRARY SEARCH; PERFORMANCE; SEPARATION; DRUGS; APPARATUS; TLC;
D O I：
10.1080/10826076.2017.1298178
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Pressurized planar electrochromatography (PPEC) was applied to support qualitative toxicological chemical analysis performed with thin-layer chromatography (TLC) and UV-Vis spectrometry. Based on retention/migration distance data of substances obtained in TLC and PPEC systems and database of their wavelength maxima of remission UV-Vis spectra as well, a combined fit factor was calculated for substance identification. The involvement of PPEC, TLC, and UV-Vis spectral data together in calculation of the combined fit factor lead to its lower values for substances, which were not identical with reference, in comparison with those when the combined fit factor was calculated using TLC and spectral data only. The results evidence that involvement of PPEC data in qualitative toxicological chemical analysis performed with TLC and UV-Vis spectrometry enhances reliability of it. [GRAPHICS] .
引用
收藏
页码：320 / 326
页数：7
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