Synthetic riboswitches - A tool comes of age

被引：74

作者：

Groher, Florian ^{[1
]}

Suess, Beatrix ^{[1
]}

机构：

[1] Tech Univ Darmstadt, Dept Biol, D-64287 Darmstadt, Germany

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS | 2014年 / 1839卷 / 10期

关键词：

Synthetic biology; Engineered riboswitch; Aptamer; Theophylline; Tetracycline; Ribozyme; MAMMALIAN GENE-EXPRESSION; SACCHAROMYCES-CEREVISIAE; RNA APTAMER; TRANSLATIONAL INITIATION; MOLECULAR DISCRIMINATION; CONFORMATIONAL CAPTURE; SECONDARY STRUCTURE; CONDITIONAL CONTROL; RATIONAL DESIGN; BINDING;

D O I：

10.1016/j.bbagrm.2014.05.005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Within the last decade, it has become obvious that RNA plays an important role in regulating gene expression. This has led to a plethora of approaches aiming at exploiting the outstanding chemical properties of RNA to develop synthetic RNA regulators for conditional gene expression systems. Consequently, many different regulators have been developed to act on various stages of gene expression. They can be engineered to respond to almost any ligand of choice and are, therefore, of great interest for applications in synthetic biology. This review presents an overview of such engineered riboswitches, discusses their applicability and points out recent trends in their development. This article is part of a Special Issue entitled: Riboswitches. (c) 2014 Elsevier B.V. All rights reserved.

引用

页码：964 / 973

页数：10

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