Cloning and characterization of a calcium-binding, histamine-releasing protein from Schistosoma mansoni

被引:82
|
作者
Rao, KVN [1 ]
Chen, L [1 ]
Gnanasekar, M [1 ]
Ramaswamy, K [1 ]
机构
[1] Univ Illinois, Coll Med, Dept Biomed Sci, Rockford, IL 61107 USA
关键词
D O I
10.1074/jbc.M204114200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A homologue of the mammalian translationally controlled tumor protein (TCTP) was cloned from the human parasite Schistosoma mansoni (SmTCTP). Sequence analysis showed that SmTCTP differed from other reported TCTPs in having only one signature sequence. Subsequently, SmTCTP was cloned in a T7 expression system and expressed as a histidine-tagged fusion protein. Recombinant SmTCTP (rSmTCTP) has a molecular mass of similar to23 kDa with the histidine tag. Further analysis showed that SmTCTP transcripts and protein are expressed in all life cycle stages of the parasite within the vertebrate hosts. Interestingly, antibodies to SmTCTP were present in the sera of mice 9 weeks after infection with S. mansoni. Characterization studies showed that rSmTCTP is a calcium-binding protein that can cause histamine release from basophil/mast cells and induce eosinophil infiltration. These findings suggest that SmTCTP may have an important role in the development of allergic inflammatory responses associated with schistosomiasis and may be a target for new drug development.
引用
收藏
页码:31207 / 31213
页数:7
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