Label-Free Logic Modules and Two-Layer Cascade Based on Stem-Loop Probes Containing a G-Quadruplex Domain

被引：23

作者：

Guo, Yahui ^{[1
,2
]}

Cheng, Junjie ^{[1
]}

Wang, Jine ^{[1
]}

Zhou, Xiaodong ^{[2
]}

Hu, Jiming ^{[2
]}

Pei, Renjun ^{[1
]}

机构：

[1] Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Collaborat Innovat Ctr Suzhou Nano Sci & Technol, Suzhou Key Lab Nanotheranost,Div Nanobiomed, Suzhou 215123, Peoples R China

[2] Wuhan Univ, Coll Chem & Mol Sci, Minist Educ, Key Lab Analyt Chem Biol & Med, Wuhan 430072, Peoples R China

来源：

CHEMISTRY-AN ASIAN JOURNAL | 2014年 / 9卷 / 09期

基金：

中国国家自然科学基金;

关键词：

DNA computing; G quadruplexes; label-free detection; logic gates; multiple-input cascade; DNA STRAND DISPLACEMENT; SWITCH-ON PROBE; MOLECULAR LOGIC; CIRCUIT; COMPUTATION; GATES; NANOSTRUCTURES; NANOTECHNOLOGY; FLUORESCENCE; PLATFORM;

D O I：

10.1002/asia.201402199

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A simple, versatile, and label-free DNA computing strategy was designed by using toehold-mediated strand displacement and stem-loop probes. A full set of logic gates (YES, NOT, OR, NAND, AND, INHIBIT, NOR, XOR, XNOR) and a two-layer logic cascade were constructed. The probes contain a G-quadruplex domain, which was blocked or unfolded through inputs initiating strand displacement and the obviously distinguishable light-up fluorescent signal of G-quadruplex/NMM complex was used as the output readout. The inputs are the disease-specific nucleotide sequences with potential for clinic diagnosis. The developed versatile computing system based on our label-free and modular strategy might be adapted in multi-target diagnosis through DNA hybridization and aptamer-target interaction.

引用

页码：2397 / 2401

页数：5

共 52 条

[21] Functional Nucleic Acid Sensors [J].