pH-metric Studies and Electrochemical Behavior of Eu(III)-7carboxymethoxy-4-methylcoumarin with 5′-GMP

被引：0

作者：

Seleem, Amin A. ^{[1
,3
]}

Hussein, Belal H. M. ^{[2
,4
]}

Fathalla, Walid ^{[5
]}

Ali, Sherin A. ^{[6
]}

机构：

[1] Taibah Univ, Dept Biol, Fac Sci & Arts, Al Ula, Saudi Arabia

[2] Taibah Univ, Fac Sci & Arts, Dept Chem, Al Ula, Saudi Arabia

[3] Sohag Univ, Fac Sci, Zool Dept, Sohag, Egypt

[4] Suez Canal Univ, Dept Chem, Fac Sci, Ismailia, Egypt

[5] Port Said Univ, Dept Math & Phys Sci, Fac Engn, Port Said, Egypt

[6] Suez Canal Univ, Dept Math & Phys Sci, Fac Engn, Ismailia, Egypt

来源：

INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE | 2018年 / 13卷 / 04期

关键词：

Potentiometry; Eu(III)-7-carboxymethoxy-4-methylcoumarin (CMMC); 5 '-GMP binding properties; Voltammetry; Fluorescence spectroscopy; PERFORMANCE LIQUID-CHROMATOGRAPHY; IMPORTANT ZWITTERIONIC BUFFERS; ACETYL AMINO-ACIDS; GUANOSINE 5'-MONOPHOSPHATE; NUCLEIC-ACIDS; LANTHANIDE COMPLEXES; METAL-COMPLEXES; ENERGY-TRANSFER; DNA; NUCLEOTIDES;

D O I：

10.20964/2018.04.05

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

The interaction of Eu(III)-7-carboxymethoxy-4-methylcoumarin(CMMC) with guanosine-5'-momophosphate (5'-GMP) has been investigated using electron analytical, fluorescence, and potentiometric methods in the ethanol-water mixture solvent (0.15 volume fraction). The formation of the different binary, ternary, and quaternary complexes is confirmed by the corresponding pH-potentiometric curves. SUPERQUAD computer program has been used for the refinement of all the calculated constants in our present study. Electroanalytical techniques have been used to confirm the formation of different binary and ternary complexes under investigation. The binding constant of the ternary complex Eu(III)-CMMC-5'-GMP calculated by fluorescence technique was found to be 1.8 x 10(7) with 1:1 molar ratio. DNA cleavage properties of the CMMC, gibberellic acid, and their europium complexes were tested by gel electrophoresis.

引用

页码：3335 / 3355

页数：21