Rolling circle amplification revolutionizes diagnosis and genomics of geminiviruses

被引：319
作者：
Haible, Daniela [1 ]
Kober, Sigrid [1 ]
Jeske, Holger [1 ]
机构：
[1] Univ Stuttgart, Dept Mol Biol & Plant Virol, D-70550 Stuttgart, Germany
来源：
JOURNAL OF VIROLOGICAL METHODS | 2006年 / 135卷 / 01期
关键词：
geminivirus; diagnosis; sequencing; infectivity;
D O I：
10.1016/j.jviromet.2006.01.017
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Better, easier and cheaper than polymerase chain reaction (PCR) or antibody detection, rolling circle amplification (RCA) using the bacteriophage 29 DNA polymerase allows for a reliable diagnosis of geminiviruses and presumably all viruses with small single-stranded circular DNA genomes. The results show the efficiency of this technique in characterizing viral DNA components of several geminiviruses from experimental and natural host plant sources. The advantages are: (a) that no expensive devices are necessary, (b) simple handling, (c) detection of all infecting circular DNA components without any knowledge of sequence information in a single step, and (d) low costs per reaction. In addition, RCA-amplified viral DNA can be characterized by restriction fragment length polymorphism analysis and directly sequenced up to 900 bases in a single run circumventing cloning and plasmid purification. This shortcut will considerably accelerate genomics of at least gemini-, nano- and circoviruses in the future. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码：9 / 16
页数：8
相关论文
共 29 条
  • [1] Replicative intermediates of Tomato leaf curl virus and its satellite DNAs
    Alberter, B
    Rezaian, MA
    Jeske, H
    [J]. VIROLOGY, 2005, 331 (02) : 441 - 448
  • [2] BLANCO L, 1989, J BIOL CHEM, V264, P8935
  • [3] Briddon R. W., 1995, Bulletin OEPP, V25, P315, DOI 10.1111/j.1365-2338.1995.tb01472.x
  • [4] Diversity of DNA 1:: a satellite-like molecule associated with monopartite begomovirus-DNAβ complexes
    Briddon, RW
    Bull, SE
    Amin, I
    Mansoor, S
    Bedford, ID
    Rishi, N
    Siwatch, SS
    Zafar, Y
    Abdel-Salam, AM
    Markham, PG
    [J]. VIROLOGY, 2004, 324 (02) : 462 - 474
  • [5] Diversity of DNA β, a satellite molecule associated with some monopartite begomoviruses
    Briddon, RW
    Bull, SE
    Amin, I
    Idris, AM
    Mansoor, S
    Bedford, ID
    Dhawan, P
    Rishi, N
    Siwatch, SS
    Abdel-Salam, AM
    Brown, JK
    Zafar, Y
    Markham, PG
    [J]. VIROLOGY, 2003, 312 (01) : 106 - 121
  • [6] Rapid amplification of plasmid and phage DNA using phi29 DNA polymerase and multiply-primed rolling circle amplification
    Dean, FB
    Nelson, JR
    Giesler, TL
    Lasken, RS
    [J]. GENOME RESEARCH, 2001, 11 (06) : 1095 - 1099
  • [7] DNA B FACILITATES, BUT IS NOT ESSENTIAL FOR, THE SPREAD OF ABUTILON MOSAIC-VIRUS IN AGROINOCULATED NICOTIANA-BENTHAMIANA
    EVANS, D
    JESKE, H
    [J]. VIROLOGY, 1993, 194 (02) : 752 - 757
  • [8] CHARACTERIZATION OF BEET CURLY TOP VIRUS SUBGENOMIC DNA LOCALIZES SEQUENCES REQUIRED FOR REPLICATION
    FRISCHMUTH, T
    STANLEY, J
    [J]. VIROLOGY, 1992, 189 (02) : 808 - 811
  • [9] THE NUCLEOTIDE-SEQUENCE OF ABUTILON MOSAIC-VIRUS REVEALS PROKARYOTIC AS WELL AS EUKARYOTIC FEATURES
    FRISCHMUTH, T
    ZIMMAT, G
    JESKE, H
    [J]. VIROLOGY, 1990, 178 (02) : 461 - 468
  • [10] BEET CURLY TOP VIRUS SYMPTOM AMELIORATION IN NICOTIANA-BENTHAMIANA TRANSFORMED WITH A NATURALLY-OCCURRING VIRAL SUBGENOMIC DNA
    FRISCHMUTH, T
    STANLEY, J
    [J]. VIROLOGY, 1994, 200 (02) : 826 - 830
123