Electrosprayed poly(vinylidene fluoride) microparticles for tissue engineering applications

被引:88
作者
Correia, D. M. [1 ,2 ]
Goncalves, R. [1 ,2 ]
Ribeiro, C. [1 ,3 ]
Sencadas, V. [1 ,4 ]
Botelho, G. [2 ]
Gomez Ribelles, J. L. [5 ,6 ]
Lanceros-Mendez, S. [1 ,3 ]
机构
[1] Univ Minho, Ctr Dept Fis, P-4710057 Braga, Portugal
[2] Univ Minho, Ctr Dept Quim, P-4710057 Braga, Portugal
[3] INL Int Iberian Nanotechnol Lab, P-4715330 Braga, Portugal
[4] Inst Politecn Cavado & Ave, Escola Super Tecnol, P-4750810 Barcelos, Portugal
[5] Univ Politecn Valencia, Ctr Biomat & Tissue Engn, Valencia 46022, Spain
[6] Networking Res Ctr Bioengn Biomat & Nanomed CIBER, Valencia, Spain
关键词
BETA-PHASE; MORPHOLOGY; MICROSPHERES; PVDF; BIOMATERIALS; SCAFFOLDS;
D O I
10.1039/c4ra04581e
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Poly(vinylidene fluoride) (PVDF) microparticles have been produced by electrospraying as a suitable substrate for tissue engineering applications. The influence of the polymer solution concentration and processing parameters, such as electric field, flow rate and inner needle diameter, on microparticle size and distribution has been studied. Polymer concentration is the most influential parameter on PVDF microparticle formation. Higher concentrations promote the formation of fibers while dilute or semi dilute concentrations favor the formation of PVDF microparticles with average diameters ranging between 0.81 +/- 0.34 and 5.55 +/- 2.34 mu m. Once the formation of microparticles is achieved, no significant differences were found with the variation of other electrospray processing parameters. The electroactive beta-phase content, between 63 and 74%, and the crystalline phase content, between 45 and 55%, are mainly independent of the processing parameters. Finally, MC-3T3-E1 cell adhesion on the PVDF microparticles is assessed, indicating their potential use for biomedical applications.
引用
收藏
页码:33013 / 33021
页数:9
相关论文
共 31 条
[1]   Subcritical CO2 sintering of microspheres of different polymeric materials to fabricate scaffolds for tissue engineering [J].
Bhamidipati, Manjari ;
Sridharan, BanuPriya ;
Scurto, Aaron M. ;
Detamore, Michael S. .
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, 2013, 33 (08) :4892-4899
[2]   Electrospraying of polymers with therapeutic molecules: State of the art [J].
Bock, N. ;
Dargaville, T. R. ;
Woodruff, M. A. .
PROGRESS IN POLYMER SCIENCE, 2012, 37 (11) :1510-1551
[3]   Electrospraying, a Reproducible Method for Production of Polymeric Microspheres for Biomedical Applications [J].
Bock, Nathalie ;
Woodruff, Maria A. ;
Hutmacher, Dietmar W. ;
Dargaville, Tim R. .
POLYMERS, 2011, 3 (01) :131-149
[4]   Vibrational spectrum of PVDF and its interpretation [J].
Bormashenko, Y ;
Pogreb, R ;
Stanevsky, O ;
Bormashenko, E .
POLYMER TESTING, 2004, 23 (07) :791-796
[5]   Controlling surface morphology of electrospun polystyrene fibers: Effect of humidity and molecular weight in the electrospinning process [J].
Casper, CL ;
Stephens, JS ;
Tassi, NG ;
Chase, DB ;
Rabolt, JF .
MACROMOLECULES, 2004, 37 (02) :573-578
[6]   Elastomeric biomaterials for tissue engineering [J].
Chen, Qizhi ;
Liang, Shuling ;
Thouas, George A. .
PROGRESS IN POLYMER SCIENCE, 2013, 38 (3-4) :584-671
[7]   Controlled deposition of electrospun poly(ethylene oxide) fibers [J].
Deitzel, JM ;
Kleinmeyer, JD ;
Hirvonen, JK ;
Tan, NCB .
POLYMER, 2001, 42 (19) :8163-8170
[8]   Smart biomaterials design for tissue engineering and regenerative medicine [J].
Furth, Mark E. ;
Atala, Anthony ;
Van Dyke, Mark E. .
BIOMATERIALS, 2007, 28 (34) :5068-5073
[9]   Gelatin microparticles aggregates as three-dimensional scaffolding system in cartilage engineering [J].
Garcia Cruz, D. M. ;
Sardinha, V. ;
Escobar Ivirico, J. L. ;
Mano, J. F. ;
Gomez Ribelles, J. L. .
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, 2013, 24 (02) :503-513
[10]   Chitosan microparticles for "in vitro" 3D culture of human chondrocytes [J].
Garcia-Giralt, N. ;
Garcia Cruz, D. M. ;
Nogues, X. ;
Escobar Ivirico, J. L. ;
Gomez Ribelles, J. L. .
RSC ADVANCES, 2013, 3 (18) :6362-6368