Detection of Haemophilus influenzae and Streptococcus pneumoniae DNA in blood culture by a single PCR assay

被引：27
作者：
HassanKing, M
Baldeh, I
Adegbola, R
Omosigho, C
Usen, SO
Oparaugo, A
Greenwood, BM
机构：
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 1996年 / 34卷 / 08期
关键词：
D O I：
10.1128/JCM.34.8.2030-2032.1996
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A multiplex PCR assay was developed to screen blood cultures from children in The Gambia with suspected pneumonia for the simultaneous detection of Haemophilus influenzae type b and Streptoccus pneumoniae isolates. Analysis of 295 blood cultures showed that PCR detected the organisms in all samples positive by culture in two samples infected with H. influenzae type b and four samples infected with S. pneumoniae that were culture negative, indicating that this method is sensitive for detecting these organisms in blood cultures.
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页码：2030 / 2032
页数：3
相关论文
共 8 条
[1]   PCR FOR CAPSULAR TYPING OF HAEMOPHILUS-INFLUENZAE [J].
FALLA, TJ ;
CROOK, DWM ;
BROPHY, LN ;
MASKELL, D ;
KROLL, JS ;
MOXON, ER .
JOURNAL OF CLINICAL MICROBIOLOGY, 1994, 32 (10) :2382-2386
[2]   ETIOLOGY OF ACUTE LOWER RESPIRATORY-TRACT INFECTIONS IN GAMBIAN CHILDREN .2. ACUTE LOWER RESPIRATORY-TRACT INFECTION IN CHILDREN AGES ONE TO NINE YEARS PRESENTING AT THE HOSPITAL [J].
FORGIE, IM ;
ONEILL, KP ;
LLOYDEVANS, N ;
LEINONEN, M ;
CAMPBELL, H ;
WHITTLE, HC ;
GREENWOOD, BM .
PEDIATRIC INFECTIOUS DISEASE JOURNAL, 1991, 10 (01) :42-47
[3]   ETIOLOGY OF ACUTE LOWER RESPIRATORY-TRACT INFECTIONS IN GAMBIAN CHILDREN .1. ACUTE LOWER RESPIRATORY-TRACT INFECTIONS IN INFANTS PRESENTING AT THE HOSPITAL [J].
FORGIE, IM ;
ONEILL, KP ;
LLOYDEVANS, N ;
LEINONEN, M ;
CAMPBELL, H ;
WHITTLE, HC ;
GREENWOOD, BM .
PEDIATRIC INFECTIOUS DISEASE JOURNAL, 1991, 10 (01) :33-41
[4]   DETECTION OF STREPTOCOCCUS-PNEUMONIAE DNA IN BLOOD CULTURES BY PCR [J].
HASSANKING, M ;
BALDEH, I ;
SECKA, O ;
FALADE, A ;
GREENWOOD, B .
JOURNAL OF CLINICAL MICROBIOLOGY, 1994, 32 (07) :1721-1724
[5]   IMPORTANCE OF ENTERIC BACTERIA AS A CAUSE OF PNEUMONIA, MENINGITIS AND SEPTICEMIA AMONG CHILDREN IN A RURAL-COMMUNITY IN THE GAMBIA, WEST-AFRICA [J].
ODEMPSEY, TJD ;
MCARDLE, TF ;
LLOYDEVANS, N ;
BALDEH, I ;
LAURENCE, BE ;
SECKA, O ;
GREENWOOD, BM .
PEDIATRIC INFECTIOUS DISEASE JOURNAL, 1994, 13 (02) :122-128
[6]  
RADSTROM P, 1994, J CLIN MICROBIOL, V32, P2738
[7]   DIAGNOSIS OF ACUTE BACTERIAL PNEUMONIA IN NIGERIAN CHILDREN - VALUE OF NEEDLE ASPIRATION OF LUNG AND OF COUNTERCURRENT IMMUNOELECTROPHORESIS [J].
SILVERMAN, M ;
STRATTON, D ;
DIALLO, A ;
EGLER, LJ .
ARCHIVES OF DISEASE IN CHILDHOOD, 1977, 52 (12) :925-931
[8]  
WALL RA, 1986, B WORLD HEALTH ORGAN, V64, P553
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