CMYB1 Encoding a MYB Transcriptional Activator Is Involved in Abiotic Stress and Circadian Rhythm in Rice

被引：18

作者：

Duan, Min ^{[1
]}

Huang, Peng ^{[1
]}

Yuan, Xi ^{[1
]}

Chen, Hui ^{[1
]}

Huang, Ji ^{[1
]}

Zhang, Hongsheng ^{[1
]}

机构：

[1] Nanjing Agr Univ, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Jiangsu, Peoples R China

来源：

SCIENTIFIC WORLD JOURNAL | 2014年

基金：

美国国家科学基金会;

关键词：

TRANSGENIC ARABIDOPSIS PLANTS; RESPONSIVE GENE-EXPRESSION; ORYZA-SATIVA L; DROUGHT TOLERANCE; SALT TOLERANCE; R2R3-MYB GENE; OVEREXPRESSION; FAMILY; COLD; DEHYDRATION;

D O I：

10.1155/2014/178038

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Through analysis of cold-induced transcriptome, a novel gene encoding a putative MYB transcription factor was isolated and designated Cold induced MYB 1 (CMYB1). Tissue-specific gene expression analysis revealed that CMYB1 was highly expressed in rice stems and nodes. qRT-PCR assay indicated that CMYB1 was dramatically induced by cold stress (>100-folds) and induced by exogenous ABA and osmotic stress. Interestingly, CMYB1 showed rhythmic expression profile in rice leaves at different developmental stages. Subcellular localization assay suggested that CMYB1-GFP (green fluorescent protein) fusion protein was localized in the nuclei. Moreover, CMYB1 exhibited the transcriptional activation activity when transiently expressed in rice protoplast cells. Taken together, CMYB1 probably functions as a transcriptional activator in mediating stress and rhythm responsive gene expression in rice.

引用

页数：9

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