Expression of recombinant proteins by BEVS in a macula-like virus-free silkworm cell line

被引：7

作者：

Iwanaga, Masashi ^{[1
]}

Taikui, Keita ^{[1
]}

Uchiyama, Koudai ^{[1
]}

Katsuma, Susumu ^{[2
]}

Imanishi, Shigeo ^{[3
]}

Kawasaki, Hideki ^{[1
]}

机构：

[1] Utsunomiya Univ, Fac Agr, Dept Agrobiol & Bioresources, Utsunomiya, Tochigi 3218505, Japan

[2] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Agr & Environm Biol, Bunkyo Ku, Tokyo 1138657, Japan

[3] Natl Inst Agrobiol Sci, Genebank, Tsukuba, Ibaraki 3058602, Japan

来源：

JOURNAL OF INVERTEBRATE PATHOLOGY | 2014年 / 123卷

基金：

日本科学技术振兴机构;

关键词：

Baculovirus; Maculavirus; Nucleopolyhedrovirus; BmNPV; BmMLV; BEVS; BOMBYX-MORI; BACULOVIRUS VECTOR; INFECTION;

D O I：

10.1016/j.jip.2014.09.004

中图分类号：

Q95 [动物学];

学科分类号：

071002 ;

摘要：

We previously established the first Bombyx mori macula-like virus (BmMLV)-free cell line (BmVF cells) from a B. mori embryo. In this study, we evaluated the expression of recombinant proteins in BmVF cells using a B. mori nucleopolyhedrovirus (BmNPV)-derived expression vector. Our results showed that BmVF cells are susceptible to BmNPV, and both the promoter activity of the polyhedrin gene and the post-translated modifications of a recombinant protein are equivalent between BmMLV-negative BmVF and -positive BmN4 cells. These findings indicate that persistent infection with BmMLV has no discernible effect on BmNPV-mediated protein production in B. mori cells. (C) 2014 Elsevier Inc. All rights reserved.

引用

页码：34 / 37

页数：4

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