Recognition of the nonpolar base 4-methylindole in DNA by the DNA repair adenine glycosylase MutY

被引：25

作者：

Chepanoske, CL ^{[1
]}

Langelier, CR ^{[1
]}

Chmiel, NH ^{[1
]}

David, SS ^{[1
]}

机构：

[1] Univ Utah, Dept Chem, Salt Lake City, UT 84112 USA

来源：

ORGANIC LETTERS | 2000年 / 2卷 / 09期

关键词：

D O I：

10.1021/ol005831o

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

The DNA repair adenine glycosylase MutY efficiently recognizes 7-deaza-2'-deoxyadenosine (Z) and its nonpolar isostere 4-methylindole beta-deoxynucleoside (M) opposite 7,8-dihydro-8-oxo-2'-deoxyguanosine (OG) and G in DNA. Both wild-type and truncated MutY exhibit a 10- to 20-fold higher affinity for a duplex containing OG:M than OG:Z, More efficient recognition of M over Z by MutY may be to due the lack of hydrogen bonding with the OG that facilitates nucleotide flipping during the substrate recognition process.

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页码：1341 / 1344

页数：4

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