Multi-locus sequence typing of Treponema pallidum subsp pallidum present in clinical samples from France: Infecting treponemes are genetically diverse and belong to 18 allelic profiles

被引：30

作者：

Pospisilova, Petra ^{[1
]}

Grange, Philippe Alain ^{[2
]}

Grillova, Linda ^{[1
]}

Mikalova, Lenka ^{[1
]}

Martinet, Pervenche ^{[3
]}

Janier, Michel ^{[4
]}

Vermersch, Annie ^{[5
]}

Benhaddou, Nadjet ^{[2
,6
]}

Del Giudice, Pascal ^{[7
]}

Alcaraz, Isabelle ^{[8
]}

Truchetet, Francois ^{[9
]}

Dupin, Nicolas ^{[2
,10
]}

Smajs, David ^{[1
]}

机构：

[1] Masaryk Univ, Fac Med, Dept Biol, Brno, Czech Republic

[2] Univ Sorbonne Paris Descartes, Fac Med, Inst Cochin Equipe Batteux U1016, Lab Dermatol,CNR Syphilis, Paris, France

[3] Serv Prevent Sante Conseil Dept Bouches Rhone, Marseille, France

[4] Hop St Louis, AP HP, Ctr MST, Paris, France

[5] Hop Jean Bernard, Serv Dermatol, Valenciennes, France

[6] Grp Hosp Paris Ctr Cochin, Serv Bacteriol, Hotel Dieu Broca, Paris, France

[7] Ctr Hosp Inter Reg, Serv Dermatol Infectiol, Frejus, France

[8] Hop Dron, Serv Univ Malad Infect & Voyageur, Tourcoing, France

[9] Hop Belair, Serv Dermatol, Metz, France

[10] Hop Cochin, AP HP, Serv Dermatol Venereol, Pavillon Tarnier, Paris, France

来源：

PLOS ONE | 2018年 / 13卷 / 07期

关键词：

SYPHILIS-CAUSING STRAINS; MACROLIDE RESISTANCE; IDENTIFIED GENOTYPES; GENE; PREVALENCE; MUTATIONS; EPIDEMIOLOGY; SURVEILLANCE; ASSOCIATION; SPIROCHETE;

D O I：

10.1371/journal.pone.0201068

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Treponema pallidum subsp. pallidum, the causative agent of sexually transmitted syphilis, detected in clinical samples from France, was subjected to molecular typing using the recently developed Multilocus Sequence Typing system. The samples (n = 133) used in this study were collected from 2010-2016 from patients with diagnosed primary or secondary syphilis attending outpatient centers or hospitals in several locations in France. Altogether, 18 different allelic profiles were found among the fully typed samples (n = 112). There were five allelic variants identified for TP0136, 12 for TP0548, and eight for TP0705. Out of the identified alleles, one, seven, and three novel alleles were identified in TP0136, TP0548, and TP0705, respectively. Partial allelic profiles were obtained from 6 samples. The majority of samples (n = 110) belonged to the SS14-like cluster of TPA isolates while 7 clustered with Nichols-like isolates. Patients infected with Nichols-like samples were more often older (p = 0.041) and more often diagnosed with secondary syphilis (p = 0.033) compared to patients infected with SS14-like samples. In addition, macrolide resistance caused by the A2058G mutation was found to be associated with allelic profile 1.3.1 or with strains belonging to the 1.3.1 lineage (p<0.001). The genetic diversity among TPA strains infecting the European population was surprisingly high, which suggests that additional studies are needed to reveal the full genetic diversity of TPA pathogens infecting humans.

引用

页数：17

共 61 条

[1] Origin of modern syphilis and emergence of a pandemic Treponema pallidum cluster [J].

Arora, Natasha ;

Schuenemann, Verena J. ;

Jaeger, Guenter ;

Peltzer, Alexander ;

Seitz, Alexander ;

Herbig, Alexander ;

Strouhal, Michal ;

Grillova, Linda ;

Sanchez-Buso, Leonor ;

Kuhnert, Denise ;

Bos, Kirsten I. ;

Davis, Leyla Rivero ;

Mikalova, Lenka ;

Bruisten, Sylvia ;

Komericki, Peter ;

French, Patrick ;

Grant, Paul R. ;

Pando, Maria A. ;

Gallo Vaulet, Lucia ;

Rodriguez Fermepin, Marcelo ;

Martinez, Antonio ;

Lara, Arturo Centurion ;

Giacani, Lorenzo ;

Norris, Steven J. ;

Smajs, David ;

Bosshard, Philipp P. ;

Gonzalez-Candelas, Fernando ;

Nieselt, Kay ;

Krause, Johannes ;

Bagheri, Homayoun C. .

NATURE MICROBIOLOGY, 2017, 2 (01)

[2] Median-joining networks for inferring intraspecific phylogenies [J].

Bandelt, HJ ;

Forster, P ;

Röhl, A .

MOLECULAR BIOLOGY AND EVOLUTION, 1999, 16 (01) :37-48

[3] A novel Treponema pallidum antigen, TP0136, is an outer membrane protein that binds human fibronectin [J].

Brinkman, Mary Beth ;

McGill, Melanie A. ;

Pettersson, Jonas ;

Rogers, Arthur ;

Matejkova, Petra ;

Smajs, David ;

Weinstock, George M. ;

Norris, Steven J. ;

Palzkill, Timothy .

INFECTION AND IMMUNITY, 2008, 76 (05) :1848-1857

[4] Whole Genome Sequences of Three Treponema pallidum ssp pertenue Strains: Yaws and Syphilis Treponemes Differ in Less than 0.2% of the Genome Sequence [J].

Cejkova, Darina ;

Zobanikova, Marie ;

Chen, Lei ;

Pospisilova, Petra ;

Strouhal, Michal ;

Qin, Xiang ;

Mikalova, Lenka ;

Norris, Steven J. ;

Muzny, Donna M. ;

Gibbs, Richard A. ;

Fulton, Lucinda L. ;

Sodergren, Erica ;

Weinstock, George M. ;

Smajs, David .

PLOS NEGLECTED TROPICAL DISEASES, 2012, 6 (01)

[5] Molecular Typing of Treponema pallidum: a 5-Year Surveillance in Shanghai, China [J].

Dai, Ting ;

Li, Kang ;

Lu, Haikong ;

Gu, Xin ;

Wang, Qianqiu ;

Zhou, Pingyu .

JOURNAL OF CLINICAL MICROBIOLOGY, 2012, 50 (11) :3674-3677

[6]

Flasarova M., 2006, Epidemiologie Mikrobiologie Imunologie, V55, P105

[7] Sequencing-based Molecular Typing of Treponema pallidum Strains in the Czech Republic: All Identified Genotypes are Related to the Sequence of the SS14 Strain [J].

Flasarova, Magdalena ;

Pospisilova, Petra ;

Mikalova, Lenka ;

Valisova, Zuzana ;

Dastychova, Eliska ;

Strnadel, Radim ;

Kuklova, Ivana ;

Woznicova, Vladana ;

Zakoucka, Hana ;

Smajs, David .

ACTA DERMATO-VENEREOLOGICA, 2012, 92 (06) :669-674

[8] Molecular Typing of Treponema pallidum Clinical Strains from Lisbon, Portugal [J].

Florindo, C. ;

Reigado, V. ;

Gomes, J. P. ;

Azevedo, J. ;

Santo, I. ;

Borrego, M. J. .

JOURNAL OF CLINICAL MICROBIOLOGY, 2008, 46 (11) :3802-3803

[9] Molecular typing of Treponema pallidum isolates from Buenos Aires, Argentina: Frequent Nichols-like isolates and low levels of macrolide resistance [J].

Gallo Vaulet, Lucia ;

Grillova, Linda ;

Mikalova, Lenka ;

Casco, Ricardo ;

Rodriguez Fermepin, Marcelo ;

Pando, Maria A. ;

Smajs, David .

PLOS ONE, 2017, 12 (02)

[10] Sensitivity, specificity and likelihood ratios of PCR in the diagnosis of syphilis: a systematic review and meta-analysis [J].

Gayet-Ageron, Angele ;

Lautenschlager, Stephan ;

Ninet, Beatrice ;

Perneger, Thomas V. ;

Combescure, Christophe .

SEXUALLY TRANSMITTED INFECTIONS, 2013, 89 (03) :251-256

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