Structural characterization and antioxidant activity in vitro of polysaccharides from angelica and astragalus

被引：152
作者：
Pu, Xiuying [1 ,2 ]
Ma, Xiaolong [1 ,2 ]
Liu, Lu [1 ,2 ]
Ren, Jing [1 ,2 ]
Li, Haibing [1 ,2 ]
Li, Xiaoyue [1 ,2 ]
Yu, Shuang [1 ,2 ]
Zhang, Weijie [1 ,2 ]
Fan, Wenbo [1 ,2 ]
机构：
[1] Lanzhou Univ Technol, Coll Life Sci & Engn, Lanzhou 730050, Peoples R China
[2] Gansu Educ Dept, Key Lab Screening Evaluated & Adv Proc Tradit Chi, Lanzhou 730050, Peoples R China
来源：
CARBOHYDRATE POLYMERS | 2016年 / 137卷
基金：
中国国家自然科学基金;
关键词：
Polysaccharides; Angelica and astragalus; Purification; Characterization; Antioxidant activity; SINENSIS OLIV. DIELS; PECTIC POLYSACCHARIDE; PURIFICATION; EXTRACTION; RATS; RHAMNOGALACTURONAN; PROTECTION; MECHANISM; SUGARS; DAMAGE;
D O I：
10.1016/j.carbpol.2015.10.053
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
In the present study, structural characterization and antioxidant activity of a fraction (AAP-2A) of polysaccharides from angelica and astragalus (AAP) were investigated. Characteriztion assay showed that AAP-2A had molecular weight (Mw), root-mean square (RMS) radius and polydispersity index (M-w/M-n) of 2.252 x 10(3) kDa, 28.4 nm and 1.038, respectively. There were infrared characteristic absorption peaks of polysaccharides in FT-IR spectroscopy. AAP-2A was composed of rhamnose (Rha), galactose (Gal), arabinose (Ara) and glucose (Glc) with a molar ratio of 1:2.13:3.22:6.18 in GC analysis. Methylation analysis combined with NMR spectroscopic analysis demonstrated that a preliminary structure of AAP-2A was proposed as follows: 1,3-linked Rhap, 1,3-linked Galp, 1,3-linked Araf, 1,5-linked Araf, 1,3,5-linked Araf, 1,4-linked Glcp and 1,4,6-linked Glcp interspersed with terminal Glcp. AAP-2A exhibited a surface with a sheet-like appearance in scanning electron microscope and stronger antioxidant capacity compared with AAP. (C) 2015 Elsevier Ltd. All rights reserved.
引用
收藏
页码：154 / 164
页数：11
相关论文
共 45 条
[1]  
Anna N. K., 2013, CARBOHYD RES, V381, P138
[2]  
[Anonymous], 1998, POLYM INT
[3]   Related structures of the O-polysaccharides of Cronobacter dublinensis G3983 and G3977 containing 3-(N-acetyl-L-alanyl)amino-3,6-dideoxy-D-galactose [J].
Arbatsky, Nikolay P. ;
Wang, Min ;
Turdymuratov, Eldar M. ;
Hu, Shaohui ;
Shashkov, Alexander S. ;
Wang, Lei ;
Knirel, Yuriy A. .
CARBOHYDRATE RESEARCH, 2015, 404 :132-137
[4]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[5]   Structure of an anti-tumor polysaccharide from Angelica sinensis (Oliv.) Diels [J].
Cao, Wei ;
Li, Xiao-Qiang ;
Liu, Li ;
Yang, Tie-Hong ;
Li, Chen ;
Fan, Hui-Ting ;
Jia, Min ;
Lv, Zheng-Guang ;
Mei, Qi-Bing .
CARBOHYDRATE POLYMERS, 2006, 66 (02) :149-159
[6]   Purification, composition analysis and antioxidant activity of a polysaccharide from the fruiting bodies of Ganoderma atrum [J].
Chen, Yi ;
Xie, Ming-Yong ;
Nie, Shao-Ping ;
Li, Chang ;
Wang, Yuan-Xing .
FOOD CHEMISTRY, 2008, 107 (01) :231-241
[7]  
Cui WW, 1996, CARBOHYD RES, V292, P173
[8]   A COLORIMETRIC METHOD FOR THE DETERMINATION OF SUGARS [J].
DUBOIS, M ;
GILLES, K ;
HAMILTON, JK ;
REBERS, PA ;
SMITH, F .
NATURE, 1951, 168 (4265) :167-167
[9]   Effects of Astragalus polysaccharide liposome on lymphocyte proliferation in vitro and adjuvanticity in vivo [J].
Fan, Yunpeng ;
Hu, Yuanliang ;
Wang, Deyun ;
Liu, Jiaguo ;
Zhang, Jing ;
Zhao, Xiaojuan ;
Liu, Xu ;
Liu, Cui ;
Yuan, Ju ;
Ruan, Shiliang .
CARBOHYDRATE POLYMERS, 2012, 88 (01) :68-74
[10]   MEASUREMENT OF URONIC-ACIDS WITHOUT INTERFERENCE FROM NEUTRAL SUGARS [J].
FILISETTICOZZI, TMCC ;
CARPITA, NC .
ANALYTICAL BIOCHEMISTRY, 1991, 197 (01) :157-162
12345