High production of 2,3-butanediol from glycerol without 1,3-propanediol formation by Raoultella ornithinolytica B6

被引:24
作者
Kim, Taeyeon [1 ,2 ]
Cho, Sukhyeong [3 ]
Woo, Han Min [4 ]
Lee, Sun-Mi [1 ,5 ]
Lee, Jinwon [3 ,6 ]
Um, Youngsoon [1 ,5 ]
Seo, Jin-Ho [2 ,7 ,8 ]
机构
[1] KIST, Clean Energy Res Ctr, Seoul, South Korea
[2] Seoul Natl Univ, Collage Agr & Life Sci, Interdisciplinary Program Agr Biotechnol, Seoul, South Korea
[3] Sogang Univ, Gas Refinery R&D Ctr C1, Seoul, South Korea
[4] Sungkyunkwan Univ, Dept Food Sci & Biotechnol, Suwon, South Korea
[5] Korea Univ Sci & Technol, Clean Energy & Chem Engn, Daejeon, South Korea
[6] Sogang Univ, Dept Chem & Biomol Engn, Seoul, South Korea
[7] Seoul Natl Univ, Dept Agr Biotechnol, Seoul, South Korea
[8] Seoul Natl Univ, Ctr Food & Bioconvergence, Seoul, South Korea
基金
新加坡国家研究基金会;
关键词
2,3-Butanediol; Raoultella ornithinolytica; Fed-batch fermentation; budABC; Crude glycerol; KLEBSIELLA-OXYTOCA M1; CRUDE GLYCEROL; FERMENTATION; OPTIMIZATION; CHEMICALS; STRAIN;
D O I
10.1007/s00253-017-8094-y
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Conversion of crude glycerol derived from biodiesel processes to value-added chemicals has attracted much attention. Herein, Raoultella ornithinolytica B6 was investigated for the high production of 2,3-butanediol (2,3-BD) from glycerol without 1,3-propanediol (1,3-PD) formation, a by-product hindering 2,3-BD purification. By evaluating the effects of temperature, agitation speed, and pH control strategy, the fermentation conditions favoring 2,3-BD production were found to be 25 A degrees C, 400 rpm, and pH control with a lower limit of 5.5, respectively. Notably, significant pH fluctuations which positively affect 2,3-BD production were generated by simply controlling the lower pH limit at 5.5. In fed-batch fermentation under those conditions, R. ornithinolytica B6 produced 2,3-BD up to 79.25 g/L, and further enhancement of 2,3-BD production (89.45 g/L) was achieved by overexpressing homologous 2,3-BD synthesis genes (the budABC). When pretreated crude glycerol was used as a sole carbon source, R. ornithinolytica B6 overexpressing budABC produced 78.10 g/L of 2,3-BD with the yield of 0.42 g/g and the productivity of 0.62 g/L/h. The 2,3-BD titer, yield, and productivity values obtained in this study are the highest 2,3-BD production from glycerol among 1,3-PD synthesis-deficient 2,3-BD producers, demonstrating R. ornithinolytica B6 as a promising 2,3-BD producer from glycerol.
引用
收藏
页码:2821 / 2830
页数:10
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