A Micellar Complex of a Conjugated Polyelectrolyte for Efficient FRET to Dye-Labeled DNA

被引：14
作者：
Nayak, Rati Ranjan [1 ]
Nag, Okhil Kumar [1 ]
Kang, Mijeong [1 ]
Jin, Youngeup [2 ]
Suh, Hongsuk [2 ]
Lee, Kwanghee [3 ]
Woo, Han Young [1 ]
机构：
[1] Pusan Natl Univ, Dept Nanomat Engn BK21, Miryang 627706, South Korea
[2] Pusan Natl Univ, Dept Chem, Pusan 609735, South Korea
[3] GIST, Dept Mat Sci & Engn, Kwangju 500712, South Korea
来源：
MACROMOLECULAR RAPID COMMUNICATIONS | 2009年 / 30卷 / 08期
关键词：
conjugated polyelectrolytes; donor-acceptor systems; fluorescence; FRET; surfactants; FLUORESCENCE ENERGY-TRANSFER; SURFACTANT COMPLEXATION; AQUEOUS-SOLUTION; QUANTUM YIELD; WATER; POLYMERS; SENSORS; HYBRIDIZATION; POLYFLUORENE; COPOLYMER;
D O I：
10.1002/marc.200800763
中图分类号：
O63 [高分子化学（高聚物）];
学科分类号：
070305 ; 080501 ; 081704 ;
摘要：
A polymer-surfactant micellar complex has been studied as a fluorescence resonance energy transfer (FRET) donor to fluorescein-labeled DNA (ssDNA-Fl). In water, the molar absorptivity and fluorescence quantum efficiency of cationic poly(fluorene-co-phenylene) (c-PFP) are substantially increased in the presence of non-ionic surfactants. A TEM microscopic study shows the formation of a nanowire micellar complex of c-PFP and the surfactants. About a 400% enhancement of the FRET signal is measured in c-PFP/ssDNA-Fl with Brij 30, relative to that without surfactants. The signal amplification is successfully modulated using different types of non-ionic surfactants which perturb the complexation, fine-structure of the complex (i.e., donor-acceptor separation), and the resulting energy transfer process.
引用
收藏
页码：633 / 638
页数：6
相关论文
共 41 条
[1]   Fluorescence superquenching of conjugated polyelectrolytes: applications for biosensing and drug discovery [J].
Achyuthan, KE ;
Bergstedt, TS ;
Chen, L ;
Jones, RM ;
Kumaraswamy, S ;
Kushon, SA ;
Ley, KD ;
Lu, L ;
McBranch, D ;
Mukundan, H ;
Rininsland, F ;
Shi, X ;
Xia, W ;
Whitten, DG .
JOURNAL OF MATERIALS CHEMISTRY, 2005, 15 (27-28) :2648-2656
[2]   Effect of surfactant on water-soluble conjugated polymer used in biosensor [J].
Al Attar, Hameed A. ;
Monkman, Andy P. .
JOURNAL OF PHYSICAL CHEMISTRY B, 2007, 111 (43) :12418-12426
[3]   Fluorescent polyelectrolytes as protein sensors [J].
Ambade, Ashootosh Vasant ;
Sandanaraj, Britto Selvaraj ;
Klaikherd, Akamol ;
Thayumanavan, S. .
POLYMER INTERNATIONAL, 2007, 56 (04) :474-481
[4]  
BECHER P, 1967, NONIONIC SURFACTANTS, pCH18
[5]   Poly(aryleneethynylene)s: Syntheses, properties, structures, and applications [J].
Bunz, UHF .
CHEMICAL REVIEWS, 2000, 100 (04) :1605-1644
[6]   Interactions between surfactants and {1,4-phenylene-[9,9-bis(4-phenoxy-butylsulfonate)]fluorene-2,7-diyl} [J].
Burrows, HD ;
Lobo, VMM ;
Pina, J ;
Ramos, ML ;
de Melo, JS ;
Valente, AJM ;
Tapia, MJ ;
Pradhan, S ;
Scherf, U ;
Hintschich, SI ;
Rothe, C ;
Monkman, AP .
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 2005, 270 :61-66
[7]   Fluorescence enhancement of the water-soluble poly{1,4-phenylene-[9,9-bis(4-phenoxybutylsulfonate)]fluorene-2,7-diyl} copolymer in n-dodecylpentaoxyethylene glycol ether micelles [J].
Burrows, HD ;
Lobo, VMM ;
Pina, J ;
Ramos, ML ;
de Melo, JS ;
Valente, AJM ;
Tapia, MJ ;
Pradhan, S ;
Scherf, U .
MACROMOLECULES, 2004, 37 (20) :7425-7427
[8]   Tuning the properties of conjugated polyelectrolytes through surfactant complexation [J].
Chen, LH ;
Xu, S ;
McBranch, D ;
Whitten, D .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2000, 122 (38) :9302-9303
[9]   Design and synthesis of fluorescence "turn-on" chemosensors based on photoinduced electron transfer in conjugated polymers [J].
Fan, LJ ;
Zhang, Y ;
Jones, WE .
MACROMOLECULES, 2005, 38 (07) :2844-2849
[10]   Continuous fluorometric assays for acetylcholinesterase activity and inhibition with conjugated polyelectrolytes [J].
Feng, Fude ;
Tang, Yanli ;
Wang, Shu ;
Li, Yuliang ;
Zhu, Daoben .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2007, 46 (41) :7882-7886
12345