Phosphoinositide 3-kinase/protein kinase B (PI3K/AKT) and Janus kinase/signal transducer and activator of transcription (JAK/STAT) follicular signalling is conserved in the mare ovary
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作者:
Hall, Sally E.
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Univ Newcastle, Sch Environm & Life Sci, Prior Res Ctr Reprod Sci, Life Sci Bldg, Callaghan, NSW 2308, Australia
Univ Canberra, Invas Anim Cooperat Res Ctr, Bldg 22, Bruce, ACT 2617, AustraliaUniv Newcastle, Sch Environm & Life Sci, Prior Res Ctr Reprod Sci, Life Sci Bldg, Callaghan, NSW 2308, Australia
Hall, Sally E.
[1
,2
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Upton, Rose M. O.
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Univ Newcastle, Sch Environm & Life Sci, Prior Res Ctr Reprod Sci, Life Sci Bldg, Callaghan, NSW 2308, AustraliaUniv Newcastle, Sch Environm & Life Sci, Prior Res Ctr Reprod Sci, Life Sci Bldg, Callaghan, NSW 2308, Australia
Upton, Rose M. O.
[1
]
McLaughlin, Eileen A.
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Univ Newcastle, Sch Environm & Life Sci, Prior Res Ctr Reprod Sci, Life Sci Bldg, Callaghan, NSW 2308, Australia
Univ Auckland, Sch Biol Sci, Thomas Bldg, Auckland 1010, New ZealandUniv Newcastle, Sch Environm & Life Sci, Prior Res Ctr Reprod Sci, Life Sci Bldg, Callaghan, NSW 2308, Australia
McLaughlin, Eileen A.
[1
,3
]
Sutherland, Jessie M.
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Univ Newcastle, Sch Environm & Life Sci, Prior Res Ctr Reprod Sci, Life Sci Bldg, Callaghan, NSW 2308, Australia
Hunter Med Res Inst, Pregnancy & Reprod Program, New Lambton Hts, NSW 2305, AustraliaUniv Newcastle, Sch Environm & Life Sci, Prior Res Ctr Reprod Sci, Life Sci Bldg, Callaghan, NSW 2308, Australia
Sutherland, Jessie M.
[1
,4
]
机构:
[1] Univ Newcastle, Sch Environm & Life Sci, Prior Res Ctr Reprod Sci, Life Sci Bldg, Callaghan, NSW 2308, Australia
[2] Univ Canberra, Invas Anim Cooperat Res Ctr, Bldg 22, Bruce, ACT 2617, Australia
[3] Univ Auckland, Sch Biol Sci, Thomas Bldg, Auckland 1010, New Zealand
[4] Hunter Med Res Inst, Pregnancy & Reprod Program, New Lambton Hts, NSW 2305, Australia
The mare ovary is unique in its anatomical structure; however, the signalling pathways responsible for physiological processes, such as follicular activation, remain uncharacterised. This provided us with the impetus to explore whether signalling molecules from important folliculogenesis pathways, phosphoinositide 3-kinase/protein kinase B (PI3K/AKT) and Janus kinase/signal transducer and activator of transcription (JAK/STAT), are conserved in the mare ovary. Messenger RNA expression of six genes important in follicle development was measured using quantitative polymerase chain reaction and protein localisation of key pathway members (PI3K, AKT1, phosphatase and tensin homologue (PTEN), JAK1, STAT3 and suppressor of cytokine signalling 4 (SOCS4)) was compared in tissue from fetal and adult mare ovaries. Tissue from adult ovaries exhibited significantly increased levels of mRNA expression of PI3K, AKT1, PTEN, JAK1, STAT3 and SOCS4 compared with tissue from fetal ovaries. PI3K, AKT1, JAK1 and STAT3 demonstrated redistributed localisation, from pregranulosa cells in fetal development, to both the oocyte and granulosa cells of follicles in the adult ovary, whilst negative feedback molecules PTEN and SOCS4 were only localised to the granulosa cells in the adult ovary. These findings suggest that the PI3K/AKT and JAK/STAT signalling pathways are utilised during folliculogenesis in the mare, similarly to previously studied mammalian species, and may serve as useful biomarkers for assessment of ovary development in the horse.
机构:
Xuzhou Med Coll, Dept Infect Dis, Affiliated Hosp 1, Xuzhou 221002, Jiangsu, Peoples R ChinaXuzhou Med Coll, Dept Infect Dis, Affiliated Hosp 1, Xuzhou 221002, Jiangsu, Peoples R China
Yan, Xue-bing
Chen, Zhi
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Zhejiang Univ, Key Lab Minist Hlth, Coll Med Sci, Inst Infect Dis,Affiliated Hosp 1, Hangzhou 310003, Zhejiang, Peoples R ChinaXuzhou Med Coll, Dept Infect Dis, Affiliated Hosp 1, Xuzhou 221002, Jiangsu, Peoples R China
Chen, Zhi
Brechot, Christian
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Univ Paris Sud, Hop Paul Brousse, Ctr Hepato Biliaire, INSERM,U785, F-94804 Villejuif, FranceXuzhou Med Coll, Dept Infect Dis, Affiliated Hosp 1, Xuzhou 221002, Jiangsu, Peoples R China
机构:
Toho Univ, Sch Med, Dept Internal Med, Div Rheumatol, Tokyo 1438541, JapanToho Univ, Sch Med, Dept Internal Med, Div Rheumatol, Tokyo 1438541, Japan
Muraoka, S.
Kusunoki, N.
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Toho Univ, Sch Med, Dept Internal Med, Div Rheumatol, Tokyo 1438541, JapanToho Univ, Sch Med, Dept Internal Med, Div Rheumatol, Tokyo 1438541, Japan
Kusunoki, N.
Takahashi, H.
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Toho Univ, Sch Med, Dept Orthopaed Surg, Tokyo 1438541, JapanToho Univ, Sch Med, Dept Internal Med, Div Rheumatol, Tokyo 1438541, Japan
Takahashi, H.
Tsuchiya, K.
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Toho Univ, Sch Med, Dept Orthopaed Surg, Tokyo 1438541, JapanToho Univ, Sch Med, Dept Internal Med, Div Rheumatol, Tokyo 1438541, Japan
Tsuchiya, K.
Kawai, S.
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Toho Univ, Sch Med, Dept Internal Med, Div Rheumatol, Tokyo 1438541, JapanToho Univ, Sch Med, Dept Internal Med, Div Rheumatol, Tokyo 1438541, Japan