Phosphoinositide 3-kinase/protein kinase B (PI3K/AKT) and Janus kinase/signal transducer and activator of transcription (JAK/STAT) follicular signalling is conserved in the mare ovary

被引:27
|
作者
Hall, Sally E. [1 ,2 ]
Upton, Rose M. O. [1 ]
McLaughlin, Eileen A. [1 ,3 ]
Sutherland, Jessie M. [1 ,4 ]
机构
[1] Univ Newcastle, Sch Environm & Life Sci, Prior Res Ctr Reprod Sci, Life Sci Bldg, Callaghan, NSW 2308, Australia
[2] Univ Canberra, Invas Anim Cooperat Res Ctr, Bldg 22, Bruce, ACT 2617, Australia
[3] Univ Auckland, Sch Biol Sci, Thomas Bldg, Auckland 1010, New Zealand
[4] Hunter Med Res Inst, Pregnancy & Reprod Program, New Lambton Hts, NSW 2305, Australia
关键词
biomarker; folliculogenesis; primordial follicle; PRIMORDIAL FOLLICLES; OOCYTE GROWTH; FETAL OVARY; PTEN; EXPRESSION; RABBIT; WOMEN; INFERTILITY; INHIBITION; PROMOTES;
D O I
10.1071/RD17024
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The mare ovary is unique in its anatomical structure; however, the signalling pathways responsible for physiological processes, such as follicular activation, remain uncharacterised. This provided us with the impetus to explore whether signalling molecules from important folliculogenesis pathways, phosphoinositide 3-kinase/protein kinase B (PI3K/AKT) and Janus kinase/signal transducer and activator of transcription (JAK/STAT), are conserved in the mare ovary. Messenger RNA expression of six genes important in follicle development was measured using quantitative polymerase chain reaction and protein localisation of key pathway members (PI3K, AKT1, phosphatase and tensin homologue (PTEN), JAK1, STAT3 and suppressor of cytokine signalling 4 (SOCS4)) was compared in tissue from fetal and adult mare ovaries. Tissue from adult ovaries exhibited significantly increased levels of mRNA expression of PI3K, AKT1, PTEN, JAK1, STAT3 and SOCS4 compared with tissue from fetal ovaries. PI3K, AKT1, JAK1 and STAT3 demonstrated redistributed localisation, from pregranulosa cells in fetal development, to both the oocyte and granulosa cells of follicles in the adult ovary, whilst negative feedback molecules PTEN and SOCS4 were only localised to the granulosa cells in the adult ovary. These findings suggest that the PI3K/AKT and JAK/STAT signalling pathways are utilised during folliculogenesis in the mare, similarly to previously studied mammalian species, and may serve as useful biomarkers for assessment of ovary development in the horse.
引用
收藏
页码:624 / 633
页数:10
相关论文
共 50 条
  • [21] Baicalin Ameliorates Collagen-Induced Arthritis Through the Suppression of Janus Kinase 1 (JAK1)/Signal Transducer and Activator of Transcription 3 (STAT3) Signaling in Mice
    Wang, Chunxiao
    Song, Yong
    Wang, Xin
    Mao, Ruijun
    Song, Lijun
    MEDICAL SCIENCE MONITOR, 2018, 24 : 9213 - 9222
  • [22] Phosphoinositide 3-Kinase (PI3K) Pathway Alterations Are Associated with Histologic Subtypes and Are Predictive of Sensitivity to PI3K Inhibitors in Lung Cancer Preclinical Models
    Spoerke, Jill M.
    O'Brien, Carol
    Huw, Ling
    Koeppen, Hartmut
    Fridlyand, Jane
    Brachmann, Rainer K.
    Haverty, Peter M.
    Pandita, Ajay
    Mohan, Sankar
    Sampath, Deepak
    Friedman, Lori S.
    Ross, Leanne
    Hampton, Garret M.
    Amler, Lukas C.
    Shames, David S.
    Lackner, Mark R.
    CLINICAL CANCER RESEARCH, 2012, 18 (24) : 6771 - 6783
  • [23] Leptin receptor signaling via Janus kinase 2/Signal transducer and activator of transcription 3 impacts on ovarian cancer cell phenotypes
    Kumar, Janani
    Fang, Hao
    McCulloch, Daniel R.
    Crowley, Tamsyn
    Ward, Alister C.
    ONCOTARGET, 2017, 8 (55) : 93530 - 93540
  • [24] Tangeretin prevents prostate cancer cell proliferation and induces apoptosis via activation of Notch signalling and regulating the androgen receptor (AR) pathway and the phosphoinositide 3-kinase (PI3k)/Akt/mTOR pathways
    Guo, Jin-Jin
    Li, Ying-Jie
    Xin, Lu-Lu
    BANGLADESH JOURNAL OF PHARMACOLOGY, 2015, 10 (04) : 937 - 947
  • [25] Discovery and Optimization of a Series of Benzothiazole Phosphoinositide 3-Kinase (PI3K)/Mammalian Target of Rapamycin (mTOR) Dual Inhibitors
    D'Angelo, Noel D.
    Kim, Tae-Seong
    Andrews, Kristin
    Booker, Shon K.
    Caenepeel, Sean
    Chen, Kui
    D'Amico, Derin
    Freeman, Dan
    Jiang, Jian
    Liu, Longbin
    McCarter, John D.
    Miguel, Tisha San
    Mullady, Erin L.
    Schrag, Michael
    Subramanian, Raju
    Tang, Jin
    Wahl, Robert C.
    Wang, Ling
    Whittington, Douglas A.
    Wu, Tian
    Xi, Ning
    Xu, Yang
    Yakowec, Peter
    Yang, Kevin
    Zalameda, Leeanne P.
    Zhang, Nancy
    Hughes, Paul
    Norman, Mark H.
    JOURNAL OF MEDICINAL CHEMISTRY, 2011, 54 (06) : 1789 - 1811
  • [26] Bupivacaine modulates the apoptosis and ferroptosis in bladder cancer via phosphatidylinositol 3-kinase (PI3K)/AKT pathway
    Hao, Jianli
    Zhang, Weiqing
    Huang, Zeqing
    BIOENGINEERED, 2022, 13 (03) : 6794 - 6806
  • [27] Eukaryotic elongation factor 2 kinase promotes angiogenesis in hepatocellular carcinoma via PI3K/Akt and STAT3
    Zhou, Ying
    Li, Yaoting
    Xu, Shihao
    Lu, Jing
    Zhu, Ziyi
    Chen, Shaoli
    Tan, Yuan
    He, Peng
    Xu, Jin
    Proud, Christopher G.
    Xie, Jianling
    Shen, Kaikai
    INTERNATIONAL JOURNAL OF CANCER, 2020, 146 (05) : 1383 - 1395
  • [28] Liraglutide, a glucagon-like peptide-1 receptor agonist, facilitates osteogenic proliferation and differentiation in MC3T3-E1 cells through phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT), extracellular signal-related kinase (ERK)1/2, and cAMP/protein kinase A (PKA) signaling pathways involving β-catenin
    Wu, Xuelun
    Li, Shilun
    Xue, Peng
    Li, Yukun
    EXPERIMENTAL CELL RESEARCH, 2017, 360 (02) : 281 - 291
  • [29] cAMP Activates TRPC6 Channels via the Phosphatidylinositol 3-Kinase (PI3K)-Protein Kinase B (PKB)-Mitogen-activated Protein Kinase Kinase (MEK)-ERK1/2 Signaling Pathway
    Shen, Bing
    Kwan, Hiu-Yee
    Ma, Xin
    Wong, Ching-On
    Du, Juan
    Huang, Yu
    Yao, Xiaoqiang
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2011, 286 (22) : 19439 - 19445
  • [30] Morusin inhibits breast cancer-induced osteolysis by decreasing phosphatidylinositol 3-kinase (PI3K)-mTOR signalling
    Zhang, Long
    Li, Weibin
    Chen, Xiaohui
    Cao, Dongmin
    You, Siyuan
    Shi, Fan
    Li, Hongyu
    Song, Yabin
    Li, Na
    Akimoto, Yoshie
    Rui, Gang
    Chen, Yu
    Wu, Zuoxing
    Xu, Ren
    CHEMICO-BIOLOGICAL INTERACTIONS, 2024, 394