Fluorescence-activated cell sorting (FACS) of whole mount in situ hybridization (WISH) labelled haematopoietic cell populations in the zebrafish

被引：10
|
作者：
Dobson, Joseph T. [1 ,2 ]
Da'as, Sahar
McBride, Eileen R.
Berman, Jason N. [2 ]
机构：
[1] Dalhousie Univ, IWK Hlth Ctr, Div Pediat Hematol Oncol, Dept Pediat, Halifax, NS B3K 6R8, Canada
[2] Dalhousie Univ, Dept Microbiol & Immunol, Halifax, NS B3K 6R8, Canada
来源：
BRITISH JOURNAL OF HAEMATOLOGY | 2009年 / 144卷 / 05期
基金：
加拿大健康研究院;
关键词：
zebrafish; FACS; haematopoiesis; flow cytometry; morphology; BEHAVIOR; EMBRYO;
D O I：
10.1111/j.1365-2141.2008.07527.x
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
The zebrafish is a robust animal model for studying vertebrate haematopoiesis and immune cell interactions. However, fluorescence activated cell sorting (FACS) has been limited due to a paucity of available functional zebrafish antibodies. We have developed a technique combining FACS with whole mount in situ hybridization (WISH) that enables the sorting and examining of fixed zebrafish blood cell populations at different stages of embryonic development, providing the opportunity to correlate RNA expression data with cellular morphology.
引用
收藏
页码：732 / 735
页数：4
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