Fluorescence-activated cell sorting (FACS) of whole mount in situ hybridization (WISH) labelled haematopoietic cell populations in the zebrafish

被引：10
|
作者：
Dobson, Joseph T. [1 ,2 ]
Da'as, Sahar
McBride, Eileen R.
Berman, Jason N. [2 ]
机构：
[1] Dalhousie Univ, IWK Hlth Ctr, Div Pediat Hematol Oncol, Dept Pediat, Halifax, NS B3K 6R8, Canada
[2] Dalhousie Univ, Dept Microbiol & Immunol, Halifax, NS B3K 6R8, Canada
来源：
BRITISH JOURNAL OF HAEMATOLOGY | 2009年 / 144卷 / 05期
基金：
加拿大健康研究院;
关键词：
zebrafish; FACS; haematopoiesis; flow cytometry; morphology; BEHAVIOR; EMBRYO;
D O I：
10.1111/j.1365-2141.2008.07527.x
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
The zebrafish is a robust animal model for studying vertebrate haematopoiesis and immune cell interactions. However, fluorescence activated cell sorting (FACS) has been limited due to a paucity of available functional zebrafish antibodies. We have developed a technique combining FACS with whole mount in situ hybridization (WISH) that enables the sorting and examining of fixed zebrafish blood cell populations at different stages of embryonic development, providing the opportunity to correlate RNA expression data with cellular morphology.
引用
收藏
页码：732 / 735
页数：4
相关论文
共 50 条
  • [31] The use of fluorescence-activated cell sorting in studying plant development and environmental responses
    Carter, Anthony D.
    Bonyadi, Roxanna
    Gifford, Miriam L.
    INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY, 2013, 57 (6-8) : 545 - 552
  • [32] Enhanced lipid production in Nannochloropsis sp using fluorescence-activated cell sorting
    Doan, Thi-Thai Yen
    Obbard, Jeffrey Philip
    GLOBAL CHANGE BIOLOGY BIOENERGY, 2011, 3 (03): : 264 - 270
  • [33] A rigid microfluidic chip for high-throughput fluorescence-activated cell sorting
    Cheng, Zhen
    Zhou, Xiao
    Gu, Miao
    Deng, Juntao
    Dong, Mingyu
    Liu, Min
    SENSORS AND ACTUATORS REPORTS, 2024, 7
  • [34] Fluorescence-activated cell sorting (FACS) of osteoblasts and osteoclasts for RNA sequencing in a medaka, Oryzias latipes (Temming & Schlegel, 1846), osteoporosis model
    Buettner, A.
    Sundaram, S.
    Vyas, H.
    Yu, T.
    Mathavan, S.
    Winkler, C.
    JOURNAL OF APPLIED ICHTHYOLOGY, 2018, 34 (02) : 481 - 488
  • [35] Detection of Infectious Adenoviruses in Environmental Waters by Fluorescence-Activated Cell Sorting Assay
    Li, Dan
    He, Miao
    Jiang, Sunny C.
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2010, 76 (05) : 1442 - 1448
  • [36] Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow
    Liao, Xiaofeng
    Makris, Melissa
    Luo, Xin M.
    JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2016, (117):
  • [37] Single Cell Micro-aspiration as an Alternative Strategy to Fluorescence-activated Cell Sorting for Giant Virus Mixture Separation
    Sahmi-Bounsiar, Dehia
    Boratto, Paulo Victor de Miranda
    Oliveira, Graziele Pereira
    Khalil, Jacques Yaacoub Bou
    La Scola, Bernard
    Andreani, Julien
    JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2019, (152):
  • [38] Cultivation and characterization of planarian neuronal cells isolated by fluorescence activated cell sorting (FACS)
    Asami, M
    Nakatsuka, T
    Hayashi, T
    Kou, K
    Kagawa, H
    Agata, K
    ZOOLOGICAL SCIENCE, 2002, 19 (11) : 1257 - 1265
  • [39] Primary Cell Culture of Purified GABAergic or Glutamatergic Neurons Established Through Fluorescence-activated Cell Sorting
    Turko, Paul
    Groberman, Keenan
    Kaiser, Toralf
    Yanagawa, Yuchio
    Vida, Imre
    JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2019, (148):
  • [40] Maintaining RNA Integrity for Transcriptomic Profiling of Ex Vivo Cultured Limbal Epithelial Stem Cells after Fluorescence-Activated Cell Sorting (FACS)
    Liu, Lei
    Nielsen, Frederik Molgaard
    Riis, Simone Elkjaer
    Emmersen, Jeppe
    Fink, Trine
    Hjortdal, Jesper Ostergaard
    Bath, Chris
    Zachar, Vladimir
    BIOLOGICAL PROCEDURES ONLINE, 2017, 19
12345