Establishment and characterization of a cell line from a chemically-induced mouse hepatoblastoma

被引：4

作者：

Kobayashi, K ^{[1
]}

Sakairi, T ^{[1
]}

Goto, K ^{[1
]}

Tsuchiya, T ^{[1
]}

Sugimoto, J ^{[1
]}

Mutai, M ^{[1
]}

机构：

[1] Mitsubishi Tokyo Pharmaceut Inc, Pharmacokinet & Toxicol Lab, Yokohama Res Ctr, Aoba Ku, Yokohama, Kanagawa 2270033, Japan

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 2000年 / 62卷 / 03期

关键词：

cell line; hepatoblastoma; HGF; mouse; phenobarbital;

D O I：

10.1292/jvms.62.263

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

We established a cell line (MHB-2) from a hepatoblastoma (HB) induced by diethylnitrosamine (DEN) and sodium phenobarbital (PB) in male B6C3F(1) mice and examined the biological characteristics of MHB-2. MHB-2 cells grew as monolayers in culture and showed a spindle or polygonal shape. Immunohistochemically, the original tumor cells and MHB-2 cells were negative for keratin, alpha-fetoprotein and albumin. Electron microscopically, MHB-2 cells had irregular-shaped nuclei with prominent nucleoli, abundant free ribosomes, myelinosomes, desmosomes and surface microvilli. Growth of this cell line was significantly accelerated by hepatocyte growth factor (HGF) and expression of its receptor c-met was confirmed by the reverse transcription-polymerase chain reaction (RT-PCR). MHB-2 however, was not found to be tumorigenic when transplanted into the subcutaneous tissue of syngeneic, nude or scid mice. To our knowledge, this is the first report on the establishment of a cell line derived from a mouse HB. MHB-2 would be useful for further studies to clarify the biological characteristics of mouse HB.

引用

页码：263 / 267

页数：5

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