Antifungal activity of a virally encoded gene in transgenic wheat

被引：61
作者：
Clausen, M
Kräuter, R
Schachermayr, G
Potrykus, I
Sautter, C
机构：
[1] Swiss Fed Inst Technol, CH-8092 Zurich, Switzerland
[2] Fed Res Stn Agraecol & Agr, CH-8046 Zurich, Switzerland
来源：
NATURE BIOTECHNOLOGY | 2000年 / 18卷 / 04期
关键词：
triticum aestivum L; antifungal activity; Tilletia tritici; transformation; KP4;
D O I：
10.1038/74521
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The cDNA encoding the antifungal protein KP4 from Ustilago maydis-infecting virus was inserted behind the ubiquitin promoter of maize and genetically transferred to wheat varieties particularly susceptible to stinking smut (Tilletia tritici) disease. The transgene was integrated and inherited over several generations. Of seven transgenic lines, three showed antifungal activity against U. maydis. The antifungal activity correlated with the presence of the KP4 transgene. KP4-transgenic, soil-grown wheat plants exhibit increased endogenous resistance against stinking smut.
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收藏
页码：446 / 449
页数：4
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