Laser capture microdissection: Big data from small samples

被引:11
|
作者
Datta, Soma
Malhotra, Lavina
Dickerson, Ryan
Chaffee, Scott
Sen, Chandan K.
Roy, Sashwati [1 ]
机构
[1] Ohio State Univ, Wexner Med Ctr, Ctr Regenerat Med & Cell Based Therapies, Dept Surg, Columbus, OH 43210 USA
基金
美国国家卫生研究院;
关键词
Laser capture microdissection (LCM); Genomics; Proteomics; Transcriptomics; POLYMERASE-CHAIN-REACTION; GENE-EXPRESSION PROFILES; COMPARATIVE GENOMIC HYBRIDIZATION; MESSENGER-RNA ANALYSIS; MASS-SPECTROMETRY; ASSISTED MICRODISSECTION; MOLECULAR-ANALYSIS; PROTEOMIC ANALYSIS; TRANSCRIPTOME ANALYSIS; TISSUE PROTEOMICS;
D O I
暂无
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Any tissue is made up of a heterogeneous mix of spatially distributed cell types. In response to any (patho) physiological cue, responses of each cell type in any given tissue may be unique and cannot be homogenized across cell-types and spatial co-ordinates. For example, in response to myocardial infarction, on one hand myocytes and fibroblasts of the heart tissue respond differently. On the other hand, myocytes in the infarct core respond differently compared to those in the peri-infarct zone. Therefore, isolation of pure targeted cells is an important and essential step for the molecular analysis of cells involved in the progression of disease. Laser capture microdissection (LCM) is powerful to obtain a pure targeted cell subgroup, or even a single cell, quickly and precisely under the microscope, successfully tackling the problem of tissue heterogeneity in molecular analysis. This review presents an overview of LCM technology, the principles, advantages and limitations and its down-stream applications in the fields of proteomics, genomics and transcriptomics. With powerful technologies and appropriate applications, this technique provides unprecedented insights into cell biology from cells grown in their natural tissue habitat as opposed to those cultured in artificial petri dish conditions.
引用
收藏
页码:1255 / 1269
页数:15
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