Laser capture microdissection: Big data from small samples

被引:11
|
作者
Datta, Soma
Malhotra, Lavina
Dickerson, Ryan
Chaffee, Scott
Sen, Chandan K.
Roy, Sashwati [1 ]
机构
[1] Ohio State Univ, Wexner Med Ctr, Ctr Regenerat Med & Cell Based Therapies, Dept Surg, Columbus, OH 43210 USA
基金
美国国家卫生研究院;
关键词
Laser capture microdissection (LCM); Genomics; Proteomics; Transcriptomics; POLYMERASE-CHAIN-REACTION; GENE-EXPRESSION PROFILES; COMPARATIVE GENOMIC HYBRIDIZATION; MESSENGER-RNA ANALYSIS; MASS-SPECTROMETRY; ASSISTED MICRODISSECTION; MOLECULAR-ANALYSIS; PROTEOMIC ANALYSIS; TRANSCRIPTOME ANALYSIS; TISSUE PROTEOMICS;
D O I
暂无
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Any tissue is made up of a heterogeneous mix of spatially distributed cell types. In response to any (patho) physiological cue, responses of each cell type in any given tissue may be unique and cannot be homogenized across cell-types and spatial co-ordinates. For example, in response to myocardial infarction, on one hand myocytes and fibroblasts of the heart tissue respond differently. On the other hand, myocytes in the infarct core respond differently compared to those in the peri-infarct zone. Therefore, isolation of pure targeted cells is an important and essential step for the molecular analysis of cells involved in the progression of disease. Laser capture microdissection (LCM) is powerful to obtain a pure targeted cell subgroup, or even a single cell, quickly and precisely under the microscope, successfully tackling the problem of tissue heterogeneity in molecular analysis. This review presents an overview of LCM technology, the principles, advantages and limitations and its down-stream applications in the fields of proteomics, genomics and transcriptomics. With powerful technologies and appropriate applications, this technique provides unprecedented insights into cell biology from cells grown in their natural tissue habitat as opposed to those cultured in artificial petri dish conditions.
引用
收藏
页码:1255 / 1269
页数:15
相关论文
共 50 条
  • [1] Semiautomated Laser Capture Microdissection of Lung Adenocarcinoma Cytology Samples
    Chowdhuri, Sinchita Roy
    Hanson, Jeffrey
    Cheng, Jerome
    Rodriguez-Canales, Jaime
    Fetsch, Patricia
    Balis, Ulysses
    Filie, Armando C.
    Giaccone, Giuseppe
    Emmert-Buck, Michael R.
    Hipp, Jason D.
    ACTA CYTOLOGICA, 2012, 56 (06) : 622 - 631
  • [2] Laser capture microdissection
    EmmertBuck, MR
    Bonner, RF
    Smith, PD
    Chuaqui, RF
    Zhuang, ZP
    Goldstein, SR
    Weiss, RA
    Liotta, LA
    SCIENCE, 1996, 274 (5289) : 998 - 1001
  • [3] Laser Capture Microdissection
    Jensen, Ellen C.
    ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY, 2013, 296 (11): : 1683 - 1687
  • [4] Application of PAXgene® fixed paraffin embedded samples to laser capture microdissection
    Yamazaki, Masaki
    Suzuki, Masami
    Kato, Atsuhiko
    CANCER SCIENCE, 2018, 109 : 996 - 996
  • [5] Combined laser capture microdissection and serial analysis of gene expression from human tissue samples
    Cho-Vega, JH
    Troncoso, P
    Do, KA
    Rago, C
    Wang, XM
    Tsavachidis, S
    Medeiros, LJ
    Spurgers, K
    Logothetis, C
    McDonnell, TJ
    MODERN PATHOLOGY, 2005, 18 (04) : 577 - 584
  • [6] Laser capture microdissection in pathology
    Fend, F
    Specht, K
    Kremer, M
    Quintanilla-Martínez, L
    LASER CAPTURE MICROSCOPY AND MICRODISSECTION, 2002, 356 : 196 - 206
  • [7] Laser-capture microdissection
    Virginia Espina
    Julia D Wulfkuhle
    Valerie S Calvert
    Amy VanMeter
    Weidong Zhou
    George Coukos
    David H Geho
    Emanuel F Petricoin
    Lance A Liotta
    Nature Protocols, 2006, 1 : 586 - 603
  • [8] Laser-capture microdissection
    Espina, Virginia
    Wulfkuhle, Julia D.
    Calvert, Valerie S.
    VanMeter, Amy
    Zhou, Weidong
    Coukos, George
    Geho, David H.
    Petricoin, Emanuel F., III
    Liotta, Lance A.
    NATURE PROTOCOLS, 2006, 1 (02) : 586 - 603
  • [9] Laser capture microdissection technology
    Espina, Virginia
    Heiby, Michael
    Pierobon, Mariaelena
    Liotta, Lance A.
    EXPERT REVIEW OF MOLECULAR DIAGNOSTICS, 2007, 7 (05) : 647 - 657
  • [10] Laser capture microdissection in pathology
    Fend, F
    Raffeld, M
    JOURNAL OF CLINICAL PATHOLOGY, 2000, 53 (09) : 666 - 672