IgG antibody response against salivary gland antigens from Oestrus ovis L. larvae (Diptera: Oestridae) in experimentally and naturally infected goats

被引：9

作者：

Angulo-Valadez, C. E. ^{[2
]}

Cepeda-Palacios, R. ^{[3
]}

Ascencio, F. ^{[2
]}

Jacquiet, Ph. ^{[1
]}

Dorchies, Ph. ^{[1
]}

Ramirez-Orduna, J. M. ^{[3
]}

Lopez, M. A. ^{[3
]}

机构：

[1] UMR INRA ENVT 1225 Interact Hotes Agents Pathogen, F-31076 Toulouse 03, France

[2] Ctr Invest Biol Noroeste, La Paz 23090, BCS, Mexico

[3] Univ Autonoma Baja California, Lab Sanidad Anim, La Paz 23080, BCS, Mexico

来源：

VETERINARY PARASITOLOGY | 2009年 / 161卷 / 3-4期

关键词：

Oestrus ovis; Salivary gland antigens; IgG response; ELISA; Goats; HUMORAL IMMUNE-RESPONSE; SHEEP; OESTROSIS;

D O I：

10.1016/j.vetpar.2009.01.027

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The aims of this study were to analyze the systemic IgG responses against third-instar salivary gland (L3SG) antigens by ELISA in Oestrus ovis experimentally infected kids (EIK) and in naturally exposed adult goats (NEG). Firstly, kids (n = 4 per group) were assigned to receive intranasally 0, 12, 24, 36, and 48 first-instars in experimental infections. Blood samples were taken from EIK at Days 0, 14,42 and 67 post-infection. At necropsy (Day 67), larval number and developmental instars were recorded. In an epidemiological study, blood serum samples were collected from 448 grazing NEG (n = 20 flocks) in Baja California Sur, Mexico. Results showed that larval establishment rate was similar in EIK groups. Systemic IgG response reached the threshold after Day 42, but humoral response was not statistically different among EIK groups receiving experimental infections. In NEG, all surveyed flocks (100%) showed specific systemic IgG antibodies to L3SG antigens and the overall goat oestrosis prevalence was 59.2%. In conclusion. larval L3SG antigens were effective in detection of specific systemic IgG antibodies against O. ovis infected kids and goats by ELISA. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：356 / 359

页数：4

共 11 条

[1] Analysis of larval antigens of Oestrus ovis for the diagnosis of oestrosis by enzyme-linked immunosorbent assay [J].

Alcaide, M ;

Reina, D ;

Frontera, E ;

Navarrete, I .

MEDICAL AND VETERINARY ENTOMOLOGY, 2005, 19 (02) :151-157

[2] Epidemiology of Oestrus ovis (Linneo, 1761) infestation in goats in Spain [J].

Alcaide, M ;

Reina, D ;

Frontera, E ;

Navarrete, I .

VETERINARY PARASITOLOGY, 2005, 130 (3-4) :277-284

[3] Proteolytic activity in salivary gland products of sheep bot fly (Oestrus ovis) larvae [J].

Angulo-Valadez, C. E. ;

Cepeda-Palacios, R. ;

Ascencio, F. ;

Jacquiet, P. ;

Dorchies, P. ;

Romero, M. J. ;

Khelifa, R. M. .

VETERINARY PARASITOLOGY, 2007, 149 (1-2) :117-125

[4]

[Anonymous], STAT WIND COMP PROGR

[5] Evaluation of a direct ELISA for the serodiagnosis of Oestrus ovis infections in sheep [J].

Goddard, P ;

Bates, P ;

Webster, KA .

VETERINARY RECORD, 1999, 144 (18) :497-501

[6] Comparison of infection rates of Oestrus ovis between sheep and goats kept in mixed flocks [J].

Papadopoulos, E. ;

Prevot, F. ;

Diakou, A. ;

Dorchies, Ph. .

VETERINARY PARASITOLOGY, 2006, 138 (3-4) :382-385

[7] Comparison of Oestrus ovis metabolic and somatic antigens for the immunodiagnosis of the zoonotic myasis oestrosis by immunoenzymatic probes [J].

Sánchez-Andrade, R ;

Romero, JL ;

Suárez, JL ;

Pedreira, J ;

Díaz, P ;

Arias, M ;

Paz-Silva, A ;

Panadero, R ;

Díez-Baños, P ;

Morrondo, P ;

Scala, A .

IMMUNOLOGICAL INVESTIGATIONS, 2005, 34 (01) :91-99

[8] Analysis of the humoral immune response to Oestrus ovis in ovine [J].

Suárez, JL ;

Scala, A ;

Romero, JA ;

Paz-Silva, A ;

Pedreira, J ;

Arias, M ;

Díaz, P ;

Morrondo, P ;

Díez-Baños, P ;

Sánchez-Andrade, R .

VETERINARY PARASITOLOGY, 2005, 134 (1-2) :153-158

[9] Cellular and humoral local immune responses in sheep experimentally infected with Oestrus ovis (Diptera: Oestridae) [J].

Tabouret, G ;

Lacroux, C ;

Andreoletti, O ;

Bergeaud, JP ;

Hailu-Tolosa, Y ;

Hoste, H ;

Prevot, F ;

Grisez, C ;

Dorchies, P ;

Jacquiet, P .

VETERINARY RESEARCH, 2003, 34 (02) :231-241

[10] Oestrus ovis (Diptera: Oestridae):: sheep humoral immune response to purified excreted/secreted salivary gland 28 kDa antigen complex from second and third instar larvae [J].

Tabouret, G ;

Prevot, F ;

Bergeaud, JP ;

Dorchies, P ;

Jacquiet, P .

VETERINARY PARASITOLOGY, 2001, 101 (01) :53-66

← 1 2 →