In situ analysis of trace elements in metalloproteins of human liver by synchrotron radiation X-ray fluorescence

被引：12
作者：
Chen, CY [1 ]
Zhang, PQ
Chai, ZF
Li, GC
Huang, YY
机构：
[1] Chinese Acad Sci, Inst High Energy Phys, Lab Nucl Analyt Tech, Beijing 100080, Peoples R China
[2] Chinese Acad Sci, Inst High Energy Phys, Synchrotron Radiat Lab, Beijing 100080, Peoples R China
来源：
SCIENCE IN CHINA SERIES A-MATHEMATICS PHYSICS ASTRONOMY | 2000年 / 43卷 / 01期
基金：
中国国家自然科学基金;
关键词：
synchrotron radiation; X-ray fluorescence analysis; in situ analysis; metalloprotein; electrophoretic separation;
D O I：
10.1007/BF02903851
中图分类号：
O29 [应用数学];
学科分类号：
070104 ;
摘要：
A method using synchrotron radiation X-ray fluorescence analysis to in situ determine trace elements during protein electrophoretically separating process was established. The distribution of elements in protein bands for human liver cytosolic sample separated by SDS-PAGE was analyzed along polyacrylamide gel. The results showed that the protein fraction of peak ill in cytosol was mainly composed of metal ion Zn-associated proteins, being in agreement with that given by atomic absorption spectrometry. Thus, it demonstrated the feasibility of this novel technique for in situ analysis of trace elements in protein bands.
引用
收藏
页码：88 / 92
页数：5
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