In situ analysis of trace elements in metalloproteins of human liver by synchrotron radiation X-ray fluorescence

被引：12
|
作者：
Chen, CY [1 ]
Zhang, PQ
Chai, ZF
Li, GC
Huang, YY
机构：
[1] Chinese Acad Sci, Inst High Energy Phys, Lab Nucl Analyt Tech, Beijing 100080, Peoples R China
[2] Chinese Acad Sci, Inst High Energy Phys, Synchrotron Radiat Lab, Beijing 100080, Peoples R China
来源：
SCIENCE IN CHINA SERIES A-MATHEMATICS PHYSICS ASTRONOMY | 2000年 / 43卷 / 01期
基金：
中国国家自然科学基金;
关键词：
synchrotron radiation; X-ray fluorescence analysis; in situ analysis; metalloprotein; electrophoretic separation;
D O I：
10.1007/BF02903851
中图分类号：
O29 [应用数学];
学科分类号：
070104 ;
摘要：
A method using synchrotron radiation X-ray fluorescence analysis to in situ determine trace elements during protein electrophoretically separating process was established. The distribution of elements in protein bands for human liver cytosolic sample separated by SDS-PAGE was analyzed along polyacrylamide gel. The results showed that the protein fraction of peak ill in cytosol was mainly composed of metal ion Zn-associated proteins, being in agreement with that given by atomic absorption spectrometry. Thus, it demonstrated the feasibility of this novel technique for in situ analysis of trace elements in protein bands.
引用
收藏
页码：88 / 92
页数：5
相关论文
共 50 条
  • [1] In situ analysis of trace elements in metalloproteins of human liver by synchrotron radiation X-ray fluorescence
    陈春英
    章佩群
    柴之芳
    李光城
    黄宇营
    Science China Mathematics, 2000, (01) : 88 - 92
  • [2] In situ analysis of trace elements in metalloproteins of human liver by synchrotron radiation X-ray fluorescence
    Chunying Chen
    Peiqun Zhang
    Zhifang Chai
    Guangcheng Li
    Yuying Huang
    Science in China Series A: Mathematics, 2000, 43 : 88 - 92
  • [3] An improved method for analysis of distribution of metalloproteins in human liver cytosol by synchrotron radiation X-ray fluorescence
    Gao, YX
    Chen, CY
    Zhao, JJ
    Zhang, PQ
    Chai, ZF
    He, W
    Huang, YY
    CHINESE JOURNAL OF ANALYTICAL CHEMISTRY, 2003, 31 (04) : 395 - 398
  • [4] Analysis of trace elements in protein bands with synchrotron radiation X-ray fluorescence after electrophoresis separation
    Dong, YX
    Gao, YX
    Chen, CY
    Li, B
    Xing, L
    Yu, HW
    He, W
    Huang, YY
    Chai, ZF
    CHINESE JOURNAL OF ANALYTICAL CHEMISTRY, 2006, 34 (04) : 443 - 446
  • [5] TRACE ELEMENT QUANTIFICATION USING SYNCHROTRON RADIATION X-RAY FLUORESCENCE ANALYSIS
    Hayakawa, Shinjiro
    Iida, Atsuo
    Gohshi, Yohichi
    ANALYTICAL SCIENCES, 1991, 7 : 509 - 512
  • [6] Measurements of trace elements in melt inclusions by synchrotron radiation x-ray fluorescence microprobe techniques
    Yu, FS
    Yuan, WM
    Han, S
    Jin, K
    Huang, YY
    He, W
    Dong, JQ
    Jia, XQ
    Cao, J
    Wang, HY
    HIGH ENERGY PHYSICS AND NUCLEAR PHYSICS-CHINESE EDITION, 2004, 28 (06): : 675 - 678
  • [7] Total reflection X-ray fluorescence analysis of light elements with synchrotron radiation and special X-ray tubes
    Streli, C
    Wobrauschek, P
    Bauer, V
    Kregsamer, P
    Gorgl, R
    Pianetta, P
    Ryon, R
    Pahlke, S
    Fabry, L
    SPECTROCHIMICA ACTA PART B-ATOMIC SPECTROSCOPY, 1997, 52 (07) : 861 - 872
  • [8] X-ray fluorescence analysis of heavy elements with a portable synchrotron
    Yamada, H
    Saisho, H
    Hirai, T
    Hirano, J
    SPECTROCHIMICA ACTA PART B-ATOMIC SPECTROSCOPY, 2004, 59 (08) : 1323 - 1328
  • [9] Analysis of trace Co in synthetic diamonds using synchrotron radiation excited X-ray fluorescence analysis
    Hayakawa, S
    Jia, XP
    Wakatsuki, M
    Gohshi, Y
    Hirokawa, T
    JOURNAL OF CRYSTAL GROWTH, 2000, 210 (1-3) : 388 - 394
  • [10] X-ray fluorescence analysis using synchrotron radiation (SYXRF)
    Haller, M
    Knochel, A
    JOURNAL OF TRACE AND MICROPROBE TECHNIQUES, 1996, 14 (03): : 461 - 488
12345