Antiviral activity of glucosylceramides isolated from Fusarium oxysporum against Tobacco mosaic virus infection

被引:12
作者
Bernardino, Mariana C. [1 ]
Couto, Michel Leon C. O. [1 ]
Vaslin, Maite F. S. [2 ]
Barreto-Bergter, Eliana [1 ]
机构
[1] Univ Fed Rio de Janeiro, Inst Microbiol Paulo Goes IMPG, Dept Microbiol Geral, Rio De Janeiro, Brazil
[2] Univ Fed Rio de Janeiro, Inst Microbiol Paulo Goes IMPG, Dept Virol, Rio De Janeiro, Brazil
来源
PLOS ONE | 2020年 / 15卷 / 11期
关键词
INDUCED RESISTANCE; DEFENSE RESPONSE; CELL-DEATH; PLANT; GLYCOPROTEIN; ELICITORS; PROTEINS; DISEASES;
D O I
10.1371/journal.pone.0242887
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Natural elicitors derived from pathogenic microorganisms represent an ecologic strategy to achieve resistance in plants against diseases. Glucosylceramides (GlcCer) are classified as neutral glycosphingolipids. GlcCer were isolated and purified from Fusarium oxysporum mycelium. F. oxysporum is a plant pathogenic fungus, abundant in soil and causing severe losses in economically important crops such as corn, tobacco, banana, cotton and passion fruit. In this study we evaluate the capacity of GlcCer in inducing resistance in N. tabacum cv Xanthi plants against Tobacco mosaic virus (TMV). Spraying tobacco plants with GlcCer before virus infection reduced the incidence of necrotic lesions caused by TMV. In addition, plants already infected with the virus showed a reduction in hypersensitive response (HR) lesions after GlcCer treatment, suggesting an antiviral effect of GlcCer. Our investigations showed that GlcCer stimulates the early accumulation of H2O2 and superoxide radicals. In addition, the expression of PR-1 (pathogenesis-related 1, with suggested antifungal action), PR-2 (beta-1,3-glucanase), PR-3 (Chitinase), PR-5 (Osmotin), PAL (Phenylalanine ammonia-lyase), LOX (Lipoxygenase) and POX (Peroxidase) genes was highly induced after treatment of tobacco plants with GlcCer and induction levels remained high throughout a period of 6 to 120 hours. Our experiments demonstrate that GlcCer induces resistance in tobacco plants against infection by TMV.
引用
收藏
页数:20
相关论文
共 50 条
[41]   Pseudorecombination of Potato Yellow Mosaic Virus with Begomoviruses Isolated from Weeds [J].
Garcia-Torres, Alexandra ;
Lopez-Lopez, Karina ;
Vaca-Vaca, Juan Carlos .
ACTA BIOLOGICA COLOMBIANA, 2023, 28 (03) :460-471
[42]   INVITRO INHIBITORY ACTIVITY OF XYLEM EXUDATES FROM CUCURBITS TOWARDS FUSARIUM-OXYSPORUM MICROCONIDIA [J].
BILES, CL ;
MARTYN, RD ;
NETZER, D .
PHYTOPARASITICA, 1990, 18 (01) :41-49
[43]   Antiviral activity and possible mechanisms of action of pentagalloylglucose (PGG) against influenza A virus [J].
Liu, Ge ;
Xiong, Sheng ;
Xiang, Yang-Fei ;
Guo, Chao-Wan ;
Ge, Feng ;
Yang, Chong-Ren ;
Zhang, Ying-Jun ;
Wang, Yi-Fei ;
Kitazato, Kaio .
ARCHIVES OF VIROLOGY, 2011, 156 (08) :1359-1369
[44]   Selection of an aptamer against rabies virus: A new class of molecules with antiviral activity [J].
Liang, Hong-Ru ;
Hu, Gui-Qiu ;
Xue, Xiang-Hong ;
Li, Lu ;
Zheng, Xue-Xing ;
Gao, Yu-Wei ;
Yang, Song-Tao ;
Xia, Xian-Zhu .
VIRUS RESEARCH, 2014, 184 :7-13
[45]   Citral induces plant systemic acquired resistance against tobacco mosaic virus and plant fungal diseases [J].
Jiang, Yue ;
Ji, Xiaolan ;
Zhang, Yueyang ;
Pan, Xiaoyu ;
Yang, Yizhou ;
Li, Yiming ;
Guo, Wenhui ;
Wang, Yong ;
Ma, Zhiqing ;
Lei, Bin ;
Yan, He ;
Liu, Xili .
INDUSTRIAL CROPS AND PRODUCTS, 2022, 183
[46]   A novel protein elicitor PeFOC1 from Fusarium oxysporum triggers defense response and systemic resistance in tobacco [J].
Li, Songwei ;
Nie, Haizhen ;
Qiu, Dewen ;
Shi, Mingwang ;
Yuan, Qianhua .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2019, 514 (04) :1074-1080
[47]   Grouper MAVS functions as a crucial antiviral molecule against nervous necrosis virus infection [J].
Huang, Youhua ;
Zhang, Jingcheng ;
Ouyang, Zhengliang ;
Liu, Jiaxin ;
Zhang, Ya ;
Hu, Yin ;
Huang, Xiaohong ;
Qin, Qiwei .
FISH & SHELLFISH IMMUNOLOGY, 2018, 72 :14-22
[48]   From Contagium vivum fluidum to Riboviria: A Tobacco Mosaic Virus-Centric History of Virus Taxonomy [J].
Zerbini, F. Murilo ;
Kitajima, Elliot W. .
BIOMOLECULES, 2022, 12 (10)
[49]   Antiviral activity of rChIFN-α against vesicular stomatitis virus and Newcastle disease virus: A novel recombinant chicken interferon-α showed high antiviral activity [J].
Hou, Fengxiang ;
Liu, Ke ;
Shen, Ting ;
Zhou, Bin ;
Cao, Ruibin ;
Li, Peide ;
Chen, Puyan .
RESEARCH IN VETERINARY SCIENCE, 2011, 91 (03) :E73-E79
[50]   Brachypodium Phenylalanine Ammonia Lyase (PAL) Promotes Antiviral Defenses against Panicum mosaic virus and Its Satellites [J].
Pant, Shankar R. ;
Irigoyen, Sonia ;
Liu, Jiaxing ;
Bedre, Renesh ;
Christensen, Shawn A. ;
Schmelz, Eric A. ;
Sedbrook, John C. ;
Scholthof, Karen-Beth G. ;
Mandadi, Kranthi K. .
MBIO, 2021, 12 (01) :1-17