Antiviral activity of glucosylceramides isolated from Fusarium oxysporum against Tobacco mosaic virus infection

被引：13

作者：

Bernardino, Mariana C. ^{[1
]}

Couto, Michel Leon C. O. ^{[1
]}

Vaslin, Maite F. S. ^{[2
]}

Barreto-Bergter, Eliana ^{[1
]}

机构：

[1] Univ Fed Rio de Janeiro, Inst Microbiol Paulo Goes IMPG, Dept Microbiol Geral, Rio De Janeiro, Brazil

[2] Univ Fed Rio de Janeiro, Inst Microbiol Paulo Goes IMPG, Dept Virol, Rio De Janeiro, Brazil

来源：

PLOS ONE | 2020年 / 15卷 / 11期

关键词：

INDUCED RESISTANCE; DEFENSE RESPONSE; CELL-DEATH; PLANT; GLYCOPROTEIN; ELICITORS; PROTEINS; DISEASES;

D O I：

10.1371/journal.pone.0242887

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Natural elicitors derived from pathogenic microorganisms represent an ecologic strategy to achieve resistance in plants against diseases. Glucosylceramides (GlcCer) are classified as neutral glycosphingolipids. GlcCer were isolated and purified from Fusarium oxysporum mycelium. F. oxysporum is a plant pathogenic fungus, abundant in soil and causing severe losses in economically important crops such as corn, tobacco, banana, cotton and passion fruit. In this study we evaluate the capacity of GlcCer in inducing resistance in N. tabacum cv Xanthi plants against Tobacco mosaic virus (TMV). Spraying tobacco plants with GlcCer before virus infection reduced the incidence of necrotic lesions caused by TMV. In addition, plants already infected with the virus showed a reduction in hypersensitive response (HR) lesions after GlcCer treatment, suggesting an antiviral effect of GlcCer. Our investigations showed that GlcCer stimulates the early accumulation of H2O2 and superoxide radicals. In addition, the expression of PR-1 (pathogenesis-related 1, with suggested antifungal action), PR-2 (beta-1,3-glucanase), PR-3 (Chitinase), PR-5 (Osmotin), PAL (Phenylalanine ammonia-lyase), LOX (Lipoxygenase) and POX (Peroxidase) genes was highly induced after treatment of tobacco plants with GlcCer and induction levels remained high throughout a period of 6 to 120 hours. Our experiments demonstrate that GlcCer induces resistance in tobacco plants against infection by TMV.

引用

页数：20

共 50 条

[21] Screening, Diversity, and Characterization of Fungal Endophytes Isolated From the Halophyte Limonium axillare and the Potential of Biocontrol Antagonists Against Fusarium oxysporum [J].

Alhaddad, Fedae ;

Abu-Dieyeh, Mohammed ;

Jaoua, Samir ;

Al-Ghouti, Mohammad A. ;

Al-Thani, Roda ;

Ahmed, Talaat .

PLANT DIRECT, 2025, 9 (03)

[22] Ginkgolide B produced endophytic fungus (Fusarium oxysporum) isolated from Ginkgo biloba [J].

Cui, Yuna ;

Yi, Dawei ;

Bai, Xiufeng ;

Sun, Baoshan ;

Zhao, Yuqing ;

Zhang, Yixuan .

FITOTERAPIA, 2012, 83 (05) :913-920

[23] Antifungal activity of an artificial peptide aptamer SNP-D4 against Fusarium oxysporum [J].

Huang, Junjun ;

Wang, Dan ;

Li, Hong ;

Tang, Yanqiong ;

Ma, Xiang ;

Tang, Hongqian ;

Lin, Min ;

Liu, Zhu .

PEERJ, 2022, 10

[24] Antiviral Activity of Glycyrrhizin against Hepatitis C Virus In Vitro [J].

Matsumoto, Yoshihiro ;

Matsuura, Tomokazu ;

Aoyagi, Haruyo ;

Matsuda, Mami ;

Hmwe, Su Su ;

Date, Tomoko ;

Watanabe, Noriyuki ;

Watashi, Koichi ;

Suzuki, Ryosuke ;

Ichinose, Shizuko ;

Wake, Kenjiro ;

Suzuki, Tetsuro ;

Miyamura, Tatsuo ;

Wakita, Takaji ;

Aizaki, Hideki .

PLOS ONE, 2013, 8 (07)

[25] Isolation, identification, and evaluation of the biocontrol potential of a Bacillus velezensis strain against tobacco root rot caused by Fusarium oxysporum [J].

Li, X. J. ;

Yao, C. X. ;

Qiu, R. ;

Bai, J. K. ;

Liu, C. ;

Chen, Y. G. ;

Li, S. J. .

JOURNAL OF APPLIED MICROBIOLOGY, 2023, 134 (01)

[26] Inhibitory effects of sulfated lentinan with different degree of sulfation against tobacco mosaic virus (TMV) in tobacco seedlings [J].

Wang, Jie ;

Yu, Guanghong ;

Li, Yihong ;

Shen, Lili ;

Qian, Yumei ;

Yang, Jinguang ;

Wang, Fenglong .

PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY, 2015, 122 :38-43

[27] Sulfated fucan oligosaccharides elicit defense responses in tobacco and local and systemic resistance against tobacco mosaic virus [J].

Klarzynski, O ;

Descamps, V ;

Plesse, B ;

Yvin, JC ;

Kloareg, B ;

Fritig, B .

MOLECULAR PLANT-MICROBE INTERACTIONS, 2003, 16 (02) :115-122

[28] Graphene-Cu Nanocomposites Induce Tolerance against Fusarium oxysporum, Increase Antioxidant Activity, and Decrease Stress in Tomato Plants [J].

Cota-Ungson, Diana ;

Gonzalez-Garcia, Yolanda ;

Cadenas-Pliego, Gregorio ;

Alpuche-Solis, Angel Gabriel ;

Benavides-Mendoza, Adalberto ;

Juarez-Maldonado, Antonio .

PLANTS-BASEL, 2023, 12 (12)

[29] Plant-derived compounds: A potential source of drugs against Tobacco mosaic virus [J].

Cai, Lin ;

Zhang, Wang ;

Jia, Huanyu ;

Feng, Hui ;

Wei, Xuefeng ;

Chen, Haitao ;

Wang, Daibin ;

Xue, Yang ;

Sun, Xianchao .

PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY, 2020, 169

[30] Antiviral activity of phage display-selected peptides against Japanese encephalitis virus infection in vitro and in vivo [J].

Wei, Jianchao ;

Hameed, Muddassar ;

Wang, Xin ;

Zhang, Junjie ;

Guo, Shuang ;

Anwar, Muhammad Naveed ;

Pang, Linlin ;

Liu, Ke ;

Li, Beibei ;

Shao, Donghua ;

Qiu, Yafeng ;

Zhong, Dengke ;

Zhou, Bin ;

Ma, Zhiyong .

ANTIVIRAL RESEARCH, 2020, 174

← 1 2 3 4 5 →