Group 5 determination in Pooideae grass pollen extracts by monoclonal antibody-based ELISA. Correlation with biologic activity

被引:15
作者
Ramirez, J [1 ]
Obispo, TM [1 ]
Duffort, O [1 ]
Carpizo, JA [1 ]
Chamorro, MJ [1 ]
Barber, D [1 ]
Ipsen, H [1 ]
Carreira, J [1 ]
Lombardero, M [1 ]
机构
[1] ALK RES,HORSHOLM,DENMARK
关键词
allergen standardization; grass group 5 allergens; grass pollen extracts; mass units; monoclonal-antibody ELISA; Pooideae pollen allergens;
D O I
10.1111/j.1398-9995.1997.tb02151.x
中图分类号
R392 [医学免疫学];
学科分类号
100102 ;
摘要
A solid-phase, monoclonal antibody-based ELISA was set up to quantitate group 5 allergens in pollen extracts of wild and cultivated Pooideae grasses. The method was able to evaluate group 5 concentration in mass units with a sensitivity in the ng/ml range and a practical working range of 1-100 ng/ml. The group 5 ELISA was compared with rocket immunoelectrophoresis for determination of allergen levels in several Phleum pratense extracts, and a very good quantitative correlation was found (r=0.98; P<0.0001). A highly significant correlation (r>0.8) was also obtained in comparing allergenic potency determined by RAST inhibition to group 5 content in several wild and cultivated grass species. The results proved the usefulness of the method in the standardization of Pooideae pollen extracts employed in diagnosis and treatment.
引用
收藏
页码:806 / 813
页数:8
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