Single Locked Nucleic Acid-enhanced nanopore genetic discrimination of pathogenic serotypes and cancer driver mutations

被引：0
|
作者：
Tian, Kai [1 ,2 ]
Chen, Xiaowei [1 ,2 ]
Luan, Binquan [1 ,2 ]
Singh, Prashant [1 ,2 ]
Yang, Zhiyu [1 ,2 ]
Gates, Kent S. [1 ,2 ]
Lin, Mengshi [1 ,2 ]
Mustapha, Azlin [1 ,2 ]
Gu, Li-Qun [1 ,2 ]
机构：
[1] Univ Missouri, Dept Bioengn, Columbia, MO 65211 USA
[2] Univ Missouri, Dalton Cardiovasc Res Ctr, Columbia, MO 65211 USA
来源：
2018 40TH ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY (EMBC) | 2018年
关键词：
NUCLEOTIDE POLYMORPHISMS; ESCHERICHIA-COLI; LUNG-CANCER; DNA; RESOLUTION; MICRORNAS; SEQUENCE; KRAS; RNA;
D O I：
暂无
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
Rapid and accurate detection of single-nucleotide polymorphism (SNP) in pathogenic mutants is crucial for broad fields from food safety monitoring to disease diagnostics and prognosis. Here, we developed a nanopore single-molecule sensor, coupled with the locked nucleic acid (LNA) technique, to accurately discriminate SNPs for detection of Shiga toxin producing Escherichia coli (STEC) O157:H7 pathogen serotype, and cancer-derived driver mutations EGFR L858R and KRAS G12D. This sensitive method, with a simplified, low cost, easy-to-operate LNA design, can be applied in food science and medical detection that need rapid and accurate determination of genetic variations.
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收藏
页码：4492 / 4495
页数：4
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