Trans-acting siRNA-mediated repression of ETTIN and ARF4 regulates heteroblasty in Arabidopsis

被引:286
|
作者
Hunter, Christine [1 ]
Willmann, Matthew R. [1 ]
Wu, Gang [1 ]
Yoshikawa, Manabu [1 ]
de la Luz Gutierrez-Nava, Maria [1 ]
Poethig, R. Scott [1 ]
机构
[1] Univ Penn, Dept Biol, Philadelphia, PA 19104 USA
来源
DEVELOPMENT | 2006年 / 133卷 / 15期
关键词
siRNA; RNAi; heterochrony; Arabidopsis; VEGETATIVE PHASE-CHANGE; AUXIN RESPONSE FACTORS; KNOX GENE-EXPRESSION; SHOOT DEVELOPMENT; SILENCING SIGNAL; LEAF DEVELOPMENT; THALIANA; MERISTEM; ASYMMETRIC-LEAVES2; MORPHOGENESIS;
D O I
10.1242/dev.02491
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Mutations in the ARGONAUTE gene ZIPPY(ZIP)/AGO7 in Arabidopsis accelerate the juvenile-to-adult transition. A screen for mutations that suppress this precocious phenotype yielded alleles of two auxin-related transcription factors known to be upregulated in zip: ETTIN (ETT)/ARF3 and ARF4. Mutations in ETT/ARF3 and ARF4 delay the expression of adult traits, demonstrating that these genes have non-redundant roles in shoot maturation. ZIP is not generally required for the production of trans-acting (ta) siRNAs, but is required for the production and/ or stability of tasiR-ARF, a ta-siRNA that targets both ETT/ARF3 and ARF4. tasiR-ARF is absent in zip-2, and overexpression of a tasiR-ARF-insensitive form of ETT mimics the zip phenotype. We conclude that the precocious phenotype of zip is attributable to the absence of tasiR-ARF-mediated repression of ETT and ARF4. The abundance of tasiR-ARF, ETT/ARF3 and ARF4 RNA does not change during vegetative development. This result suggests that tasiR-ARF regulation establishes the threshold at which leaves respond to a temporal signal, rather than being a component of this signal.
引用
收藏
页码:2973 / 2981
页数:9
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