Decellularized dermal matrix-based photo-crosslinking hydrogels as a platform for delivery of adipose derived stem cells to accelerate cutaneous wound healing

被引：30
作者：
Bo, Qitao [2 ]
Yan, Li [4 ,5 ]
Li, Hao [2 ]
Jia, Zihao [2 ]
Zhan, Anqi [2 ]
Chen, Jie [3 ]
Yuan, Zhaoqi [3 ]
Zhang, Wei [2 ,3 ]
Gao, Bowen [3 ]
Chen, Ru [1 ]
机构：
[1] Hainan Med Univ, Hainan Gen Hosp, Dept Breast Surg, Haikou, Hainan, Peoples R China
[2] Weifang Med Univ, Inst Plast Surg, Weifang, Shandong, Peoples R China
[3] Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Dept Plast & Reconstruct Surg, Sch Med, Shanghai, Peoples R China
[4] Shanghai Jiao Tong Univ, Shanghai Ruijin Hosp, Dept Dermatol, Sch Med, Shanghai, Peoples R China
[5] Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Dept Dermatol, Sch Med, Shanghai, Peoples R China
来源：
MATERIALS & DESIGN | 2020年 / 196卷
基金：
海南省自然科学基金;
关键词：
Decellularized dermal matrix; Photo-crosslinking hydrogel; Adipose-derived stem cells; Wound healing; SKIN; TISSUE; NEOVASCULARIZATION; REGENERATION; COLLAGEN; MODEL;
D O I：
10.1016/j.matdes.2020.109152
中图分类号：
T [工业技术];
学科分类号：
08 ;
摘要：
The development of an effective delivery vehicle that gives rise to a microenvironment that promotes skin regeneration remains a challenge in the field of stem cell-based therapeutic strategy for skin tissue engineering. Here, we fabricated a photo-crosslinkable hydrogel based on a decellularized dermal matrix (DDM) combined with o nitrobenzene-modified hyaluronic acid (HA-NB). The HA-NB/DDM hydrogels can be crosslinked in situ under UV ultraviolet light irradiation and adhere strongly to the surrounding skin tissue. Results showed that nude mice treated with the HA-NB/DDM hydrogel loaded with adipose-derived stem cells (ASCs) accelerated wound healing compared with those treated with the hydrogel or ASCs alone and untreated control animals. Neoepidermal formation and angiogenesis was better in wounds treated with ASC-loaded hydrogels. Further, epidermal-dermal junctions with healthy epidermal protrusions and dermal projections were observed in wounds treated with the ASC-loaded HA-NB/DDM hydrogels. Collagen deposits resembling the natural collagen structure and arrangement were observed in wounds treated with the ASC-loaded HA-NB/DDM hydrogel. Further, ASC-loaded HA-NB/DDM hydrogels can achieve satisfactory therapeutic effects in wounds with irregular
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页数：11
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