Strongyloides stercoralis genotypes in humans in Cambodia

被引：24

作者：

Schaer, Fabian ^{[1
,2
]}

Guo, Li ^{[3
]}

Streit, Adrian ^{[3
]}

Khieu, Virak ^{[1
,2
,4
]}

Muth, Sinuon ^{[4
]}

Marti, Hanspeter ^{[2
,5
]}

Odermatt, Peter ^{[1
,2
]}

机构：

[1] Swiss Trop & Publ Hlth Inst, CH-4051 Basel, Switzerland

[2] Univ Basel, Basel, Switzerland

[3] Max Planck Inst Dev Biol, Dept Evolutionary Biol, Tubingen, Germany

[4] Minist Hlth, Natl Ctr Parasitol Entomol & Malaria Control, Phnom Penh, Cambodia

[5] Swiss Trop & Publ Hlth Inst, Med & Diagnost Dept, CH-4051 Basel, Switzerland

来源：

PARASITOLOGY INTERNATIONAL | 2014年 / 63卷 / 03期

关键词：

Strongyloides stercoralis; SSU hyper variable region I/IV; Genotype; Human; Cambodia; RIBOSOMAL DNA-SEQUENCE; NEMATODA; IDENTIFICATION; REPRODUCTION; INFECTIONS; PAPILLOSUS;

D O I：

10.1016/j.parint.2014.01.010

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Little is known about the genetic variability of the soil-transmitted nematode, Strongyloides stercoralis, in humans. We sequenced portions of the small subunit rDNA (SSU), including the hyper variable regions (HVR) I and IV from S. stercoralis larvae derived from individuals living in a rural setting in Cambodia. We identified three polymorphic positions, including a previously reported one within the HVR I. HVR IV was invariable. Six different SSU alleles existed in our sample. Although different genotypes of S. stercoralis were found in the same individuals, no heterozygous larvae were found. This indicates that there is no or very little interbreeding between the different genotypes. Further studies are needed to examine if this is because sexual reproduction, which is facultative, is rare in our study area's S. stercoralis population or because what is considered to be S. stercoralis today is actually a complex of closely related species or subspecies. (C) 2014 Elsevier Ireland Ltd. All rights reserved.

引用

页码：533 / 536

页数：4

共 24 条

[1]

Baermann G., 1917, EINFACHE METHODE AUF, V57, P41

[2] Soil-transmitted helminth infections: ascariasis, trichuriasis, and hookworm [J].

Bethony, J ;

Brooker, S ;

Albonico, M ;

Geiger, SM ;

Loukas, A ;

Diemert, D ;

Hotez, PJ .

LANCET, 2006, 367 (9521) :1521-1532

[3] A molecular evolutionary framework for the phylum Nematoda [J].

Blaxter, ML ;

De Ley, P ;

Garey, JR ;

Liu, LX ;

Scheldeman, P ;

Vierstraete, A ;

Vanfleteren, JR ;

Mackey, LY ;

Dorris, M ;

Frisse, LM ;

Vida, JT ;

Thomas, WK .

NATURE, 1998, 392 (6671) :71-75

[4] Molecular phylogenetic analysis of the genus Strongyloides and related nematodes [J].

Dorris, M ;

Viney, ME ;

Blaxter, ML .

INTERNATIONAL JOURNAL FOR PARASITOLOGY, 2002, 32 (12) :1507-1517

[5] The Strongyloides (Nematoda) of sheep and the predominant Strongyloides of cattle form at least two different, genetically isolated populations [J].

Eberhardt, Alexander G. ;

Mayer, Werner E. ;

Bonfoh, Bassirou ;

Streit, Adrian .

VETERINARY PARASITOLOGY, 2008, 157 (1-2) :89-99

[6] The free-living generation of the nematode Strongyloides papillosus undergoes sexual reproduction [J].

Eberhardt, Alexander G. ;

Mayer, Werner E. ;

Streit, Adrian .

INTERNATIONAL JOURNAL FOR PARASITOLOGY, 2007, 37 (8-9) :989-1000

[7] Molecular barcodes for soil nematode identification [J].

Floyd, R ;

Abebe, E ;

Papert, A ;

Blaxter, M .

MOLECULAR ECOLOGY, 2002, 11 (04) :839-850

[8]

GENTA RM, 1989, REV INFECT DIS, V11, P755

[9] Human strongyloidiasis [J].

Grove, DI .

ADVANCES IN PARASITOLOGY, VOL 38, 1996, 38 :251-309

[10] CHROMOSOME COMPLEMENT, GAMETOGENESIS, AND DEVELOPMENT OF STRONGYLOIDES-STERCORALIS [J].

HAMMOND, MP ;

ROBINSON, RD .

JOURNAL OF PARASITOLOGY, 1994, 80 (05) :689-695

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