Copper-Free "Click" Modification of DNA via Nitrile Oxide-Norbornene 1,3-Dipolar Cycloaddition

被引:126
作者
Gutsmiedl, Katrin [1 ]
Wirges, Christian T. [1 ]
Ehmke, Veronika [1 ]
Carell, Thomas [1 ]
机构
[1] Univ Munich, Ctr Integrat Prot Sci, Dept Chem & Biochem, D-81377 Munich, Germany
关键词
AZIDE-ALKYNE CYCLOADDITION; FREE TRIAZOLE FORMATION; BENZONITRILE OXIDE; CHEMISTRY; LIGATION; BIOCONJUGATION; NUCLEOTIDES; POLYMER; SINGLE; TOOL;
D O I
10.1021/ol9005322
中图分类号
O62 [有机化学];
学科分类号
070303 ; 081704 ;
摘要
Nitrile oxides react smoothly and rapidly with norbornene-modified DNA in a copper-free click reaction. The reaction allows high density functionalization of oligodeoxyribonucleotides (ODNs) with a large variety of molecules directly on solid supports and even in synthesizers without the need for an additional catalyst.
引用
收藏
页码:2405 / 2408
页数:4
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