Mangiferin Inhibits IL-1β-Induced Inflammatory Response by Activating PPAR-γ in Human Osteoarthritis Chondrocytes

Inflammation has been reported to play critical roles in the development of osteoarthritis. In the present study, we investigated whether mangiferin (MFN) had anti-inflammatory effects in IL-1 beta-stimulated human osteoarthritis chondrocytes. The cells were treated with various concentrations of MFN in the presence or absence of IL-1 beta. The production of MMP-1, MMP-3, PGE2, and NO was measured in this study. The expression of NF-kB and PPAR-gamma was detected by western blot analysis. MFN inhibited IL-1 beta-induced inflammatory mediators PGE2 and NO production. MFN also inhibited IL-1 beta-induced MMP1 and MMP3 production. IL-1 beta-induced NF-kB activation was significantly inhibited by MFN. In addition, MFN was found to up-regulate the expression of PPAR-gamma in human osteoarthritis chondrocytes. PPAR-gamma inhibitor GW9662 significantly reversed the anti-inflammatory effects of MFN. These results suggest that MFN inhibits IL-1 beta-induced inflammatory response in human osteoarthritis chondrocytes by activating PPAR-gamma.