RNA replication by Q beta replicase: A working model

被引：61

作者：

Brown, D ^{[1
]}

Gold, L ^{[1
]}

机构：

[1] UNIV COLORADO,DEPT MOL CELLULAR & DEV BIOL,BOULDER,CO 80309

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1996年 / 93卷 / 21期

关键词：

RNA-dependent RNA polymerase; viral replication;

D O I：

10.1073/pnas.93.21.11558

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Two classes of RNA ligands that bound to separate, high affinity nucleic acid binding sites on Q beta replicase were previously identified. RNA ligands to the two sites, referred to as site I and site II, were used to investigate the molecular mechanism of RNA replication employed by the four-subunit replicase. Replication inhibition by site I- and site II-specific ligands defined two subsets of replicatable RNAs. When provided with appropriate 3' ends, ligands to either site served as replication templates. UV crosslinking experiments revealed that site I is associated with the S1 subunit, site II with elongation factor Tu, and polymerization with the viral subunit of the holoenzyme. These results provide the framework for a three site model describing template recognition and product strand initiation by Q beta replicase.

引用

页码：11558 / 11562

页数：5

共 22 条

[11] AUTOCATALYTIC SYNTHESIS OF A VIRAL RNA IN VITRO [J].

HARUNA, I ;

SPIEGELMAN, S .

SCIENCE, 1965, 150 (3698) :884-+

[12] PHYSICAL AND FUNCTIONAL HOMOLOGY BETWEEN RIBOSOMAL-PROTEIN S1 AND INTERFERENCE FACTOR-I [J].

INOUYE, H ;

POLLACK, Y ;

PETRE, J .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1974, 45 (01) :109-117

[13] REPLICATING RNA MOLECULE SUITABLE FOR A DETAILED ANALYSIS OF EXTRACELLULAR EVOLUTION AND REPLICATION [J].

KACIAN, DL ;

SPIEGELMAN, S ;

MILLS, DR ;

KRAMER, FR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1972, 69 (10) :3038-+

[14] CHARACTERIZATION OF SUBUNITS OF QBETA REPLICASE [J].

KAMEN, R .

NATURE, 1970, 228 (5271) :527-+

[15] SUBUNIT STRUCTURE OF QBETA REPLICASE [J].

KONDO, M ;

GALLERANI, R ;

WEISSMANN, C .

NATURE, 1970, 228 (5271) :525-+

[16] INTERACTIONS OF Q-BETA REPLICASE WITH Q-BETA RNA [J].

MEYER, F ;

WEBER, H ;

WEISSMANN, C .

JOURNAL OF MOLECULAR BIOLOGY, 1981, 153 (03) :631-660

[17]

MILLS DR, 1988, J MOL BIOL, V205, P751

[18] EVOLUTION OF HOST-CELL RNA INTO EFFICIENT TEMPLATE RNA BY Q-BETA REPLICASE - THE ORIGIN OF RNA IN UNTEMPLATED REACTIONS [J].

MOODY, MD ;

BURG, JL ;

DIFRANCESCO, R ;

LOVERN, D ;

STANICK, W ;

LINGOERKE, J ;

MAHDAVI, K ;

WU, Y ;

FARRELL, MP .

BIOCHEMISTRY, 1994, 33 (46) :13836-13847

[19] DEFINING A SMALLER RNA SUBSTRATE FOR ELONGATION-FACTOR TU [J].

NAZARENKO, IA ;

UHLENBECK, OC .

BIOCHEMISTRY, 1995, 34 (08) :2545-2552

[20]

WAHBA AJ, 1974, J BIOL CHEM, V249, P3314

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