Effects of polycyclic aromatic hydrocarbons on microbial community structure and PAH ring hydroxylating dioxygenase gene abundance in soil

被引:73
作者
Sawulski, Przemyslaw [1 ,2 ]
Clipson, Nicholas [1 ,2 ]
Doyle, Evelyn [1 ,2 ]
机构
[1] Univ Coll Dublin, Sch Biol & Environm Sci, Environm Microbiol Grp, Dublin 4, Ireland
[2] Univ Coll Dublin, Earth Inst, Dublin 4, Ireland
关键词
Polycyclic aromatic hydrocarbons; Degradation; Microbial community; PAH ring hydroxylating dioxygenase; TRFLP; ARISA; qPCR; PHENANTHRENE DEGRADATION; BIODEGRADATION; BIOREMEDIATION; METABOLISM; DIVERSITY; RNA; QUANTIFICATION; FLUORANTHENE; NAPHTHALENE; SEQUENCES;
D O I
10.1007/s10532-014-9703-4
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Development of successful bioremediation strategies for environments contaminated with recalcitrant pollutants requires in-depth knowledge of the microorganisms and microbial processes involved in degradation. The response of soil microbial communities to three polycyclic aromatic hydrocarbons, phenanthrene (3-ring), fluoranthene (4-ring) and benzo(a)pyrene (5-ring), was examined. Profiles of bacterial, archaeal and fungal communities were generated using molecular fingerprinting techniques (TRFLP, ARISA) and multivariate statistical tools were employed to interpret the effect of PAHs on community dynamics and composition. The extent and rate of PAH removal was directly related to the chemical structure, with the 5-ring PAH benzo(a)pyrene degraded more slowly than phenathrene or fluoranthene. Bacterial, archaeal and fungal communities were all significantly affected by PAH amendment, time and their interaction. Based on analysis of clone libraries, Actinobacteria appeared to dominate in fluoranthene amended soil, although they also represented a significant portion of the diversity in phenanthrene amended and unamended soils. In addition there appeared to be more gamma-Proteobacteria and less Bacteroidetes in soil amended with either PAH compared to the control. The soil bacterial community clearly possessed the potential to degrade PAHs as evidenced by the abundance of PAH ring hydroxylating (PAH-RHD alpha) genes from both gram negative (GN) and gram positive (GP) bacteria in PAH-amended and control soils. Although the dioxygenase gene from GP bacteria was less abundant in soil than the gene associated with GN bacteria, significant (p < 0.001) increases in the abundance of the GP PAH-RHD alpha gene were observed during phenanthrene and fluoranthene degradation, whereas there was no significant difference in the abundance of the GN PAH-RHD alpha gene during the course of the experiment. Few studies to-date have examined the effect of pollutants on more than one microbial community in soil. The current study provides information on the response of soil bacterial, archaeal and fungal communities during the degradation of three priority pollutants and contributes to a knowledge base that can inform the development of effective bioremediation strategies for contaminated sites.
引用
收藏
页码:835 / 847
页数:13
相关论文
共 61 条
[1]   Biodegradation of crude oil and pure hydrocarbons by extreme halophilic archaea from hypersaline coasts of the Arabian Gulf [J].
Al-Mailem, D. M. ;
Sorkhoh, N. A. ;
Al-Awadhi, H. ;
Eliyas, M. ;
Radwan, S. S. .
EXTREMOPHILES, 2010, 14 (03) :321-328
[2]  
[Anonymous], 2005, CERCLA PRIOR LIST HA
[3]   DEGRADATION OF PHENANTHRENE, FLUORENE, FLUORANTHENE, AND PYRENE BY A MYCOBACTERIUM SP [J].
BOLDRIN, B ;
TIEHM, A ;
FRITZSCHE, C .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (06) :1927-1930
[4]   Real-Time PCR quantification of PAH-ring hydroxylating dioxygenase (PAH-RHDα) genes from Gram positive and Gram negative bacteria in soil and sediment samples [J].
Cebron, Aurelie ;
Norini, Marie-Paule ;
Beguiristain, Thierry ;
Leyval, Corinne .
JOURNAL OF MICROBIOLOGICAL METHODS, 2008, 73 (02) :148-159
[5]   Root exudates modify bacterial diversity of phenanthrene degraders in PAH-polluted soil but not phenanthrene degradation rates [J].
Cebron, Aurelie ;
Louvel, Brice ;
Faure, Pierre ;
France-Lanord, Christian ;
Chen, Yin ;
Murrell, J. Colin ;
Leyval, Corinne .
ENVIRONMENTAL MICROBIOLOGY, 2011, 13 (03) :722-736
[6]   Influence of Vegetation on the In Situ Bacterial Community and Polycyclic Aromatic Hydrocarbon (PAH) Degraders in Aged PAH-Contaminated or Thermal-Desorption-Treated Soil [J].
Cebron, Aurelie ;
Beguiristain, Thierry ;
Faure, Pierre ;
Norini, Marie-Paule ;
Masfaraud, Jean-Francois ;
Leyval, Corinne .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2009, 75 (19) :6322-6330
[7]  
Cerniglia C.E., 1993, BIODEGRADATION, V4, P351
[8]  
Cerniglia Carl E., 1993, Current Opinion in Biotechnology, V4, P331, DOI 10.1007/BF00129093
[9]   FUNGAL METABOLISM AND DETOXIFICATION OF POLYCYCLIC AROMATIC-HYDROCARBONS [J].
CERNIGLIA, CE ;
WHITE, GL ;
HEFLICH, RH .
ARCHIVES OF MICROBIOLOGY, 1985, 143 (02) :105-110
[10]   Polycyclic aromatic hydrocarbon (PAH) degradation coupled to methanogenesis [J].
Chang, W ;
Um, Y ;
Holoman, TRP .
BIOTECHNOLOGY LETTERS, 2006, 28 (06) :425-430