Evaluation of capillary electrophoresis with post-column derivatization and laser-induced fluorescence detection for the determination of substance P and its metabolites

被引：61
作者：
Kostel, KL
Lunte, SM
机构：
[1] UNIV KANSAS,DEPT PHARMACEUT CHEM,LAWRENCE,KS 66047
[2] UNIV KANSAS,CTR BIOANALYT RES,LAWRENCE,KS 66047
来源：
JOURNAL OF CHROMATOGRAPHY B | 1997年 / 695卷 / 01期
关键词：
substance P; naphthalene-2,3-dicarboxaldehyde; beta-mercaptoethanol;
D O I：
10.1016/S0378-4347(97)00173-4
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A method for the detection of substance P and its metabolites using capillary electrophoresis with post-capillary derivatization and laser-induced fluorescence detection is described. The post-capillary chemical derivatization system employs naphthalene-2,3-dicarboxaldehyde and beta-mercaptoethanol. Two reactor designs were evaluated for the determination of substance P and its metabolites. The fluorescent spectroscopic properties of the derivatives under optimal separation conditions were also examined. The final system was evaluated for the investigation of substance P metabolism in brain following perfusion of the striatum with substance P using microdialysis sampling.
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页码：27 / 38
页数：12
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