Analysis of RNA-Protein Interactions by Cell Mixing

被引：2

作者：

Stubbs, Sarah H. ^{[1
]}

Conrad, Nicholas K. ^{[1
]}

机构：

[1] Univ Texas SW Med Ctr Dallas, Dept Microbiol, Dallas, TX 75390 USA

来源：

LABORATORY METHODS IN ENZYMOLOGY: PROTEIN, PT B | 2014年 / 539卷

关键词：

D O I：

10.1016/B978-0-12-420120-0.00005-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

RNA-protein complexes are critical for almost all aspects of gene expression. Analysis of RNA-protein interactions can be complicated by the disruption of native complexes and the formation of new, reassorted complexes upon cell lysis. Before concluding that a specific RNA and protein interact in vivo, cell-mixing experiments can be performed to ensure that observed RNA-protein complexes are not formed after lysis of cells.

引用

页码：67 / 80

页数：14

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