Analysis of RNA-Protein Interactions by Cell Mixing

被引：2

作者：

Stubbs, Sarah H. ^{[1
]}

Conrad, Nicholas K. ^{[1
]}

机构：

[1] Univ Texas SW Med Ctr Dallas, Dept Microbiol, Dallas, TX 75390 USA

来源：

LABORATORY METHODS IN ENZYMOLOGY: PROTEIN, PT B | 2014年 / 539卷

关键词：

D O I：

10.1016/B978-0-12-420120-0.00005-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

RNA-protein complexes are critical for almost all aspects of gene expression. Analysis of RNA-protein interactions can be complicated by the disruption of native complexes and the formation of new, reassorted complexes upon cell lysis. Before concluding that a specific RNA and protein interact in vivo, cell-mixing experiments can be performed to ensure that observed RNA-protein complexes are not formed after lysis of cells.

引用

页码：67 / 80

页数：14

共 50 条

[11] Mapping of RNA-protein interactions
Gopinath, Subash Chandra Bose
ANALYTICA CHIMICA ACTA, 2009, 636 (02) : 117 - 128
[12] Looking into RNA-protein interactions
不详
JOURNAL OF BIOPHOTONICS, 2009, 2 (05) : 273 - 274
[13] Cooperativity in RNA-Protein Interactions: Global Analysis of RNA Binding Specificity
Campbell, Zachary T.
Bhimsaria, Devesh
Valley, Cary T.
Rodriguez-Martinez, Jose A.
Menichelli, Elena
Williamson, James R.
Ansari, Aseem Z.
Wickens, Marvin
CELL REPORTS, 2012, 1 (05): : 570 - 581
[14] Transient RNA-protein interactions in RNA folding
Doetsch, Martina
Schroeder, Renee
Fuertig, Boris
FEBS JOURNAL, 2011, 278 (10) : 1634 - 1642
[15] Methods to study the RNA-protein interactions
V. V. Popova
M. M. Kurshakova
D. V. Kopytova
Molecular Biology, 2015, 49 : 418 - 426
[16] Methods to study RNA-protein interactions
Ramanathan, Muthukumar
Porter, Douglas F.
Khavari, Paul A.
NATURE METHODS, 2019, 16 (03) : 225 - 234
[17] RNA-protein interactions in an unstructured context
Zagrovic, Bojan
Bartonek, Lukas
Polyansky, Anton A.
FEBS LETTERS, 2018, 592 (17): : 2901 - 2916
[18] RNA-protein interactions in spherical viruses
Bink, HHJ
Pleij, CWA
ARCHIVES OF VIROLOGY, 2002, 147 (12) : 2261 - 2279
[19] Transient DNA/RNA-protein interactions
Blanco, Francisco J.
Montoya, Guillermo
FEBS JOURNAL, 2011, 278 (10) : 1643 - 1650
[20] A Global View of RNA-Protein Interactions
不详
CELL, 2012, 149 (07) : 1415 - 1415

← 1 2 3 4 5 →