Analysis of RNA-Protein Interactions by Cell Mixing

被引：2
|
作者：
Stubbs, Sarah H. [1 ]
Conrad, Nicholas K. [1 ]
机构：
[1] Univ Texas SW Med Ctr Dallas, Dept Microbiol, Dallas, TX 75390 USA
来源：
LABORATORY METHODS IN ENZYMOLOGY: PROTEIN, PT B | 2014年 / 539卷
关键词：
D O I：
10.1016/B978-0-12-420120-0.00005-0
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
RNA-protein complexes are critical for almost all aspects of gene expression. Analysis of RNA-protein interactions can be complicated by the disruption of native complexes and the formation of new, reassorted complexes upon cell lysis. Before concluding that a specific RNA and protein interact in vivo, cell-mixing experiments can be performed to ensure that observed RNA-protein complexes are not formed after lysis of cells.
引用
收藏
页码：67 / 80
页数：14
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