Quantification of neuramidase (NA) protein content

被引:9
作者
Aymard, M [1 ]
机构
[1] Univ Lyon 1, Fac Med, F-69373 Lyon 08, France
关键词
influenza; neuraminidase; immunocapture; monoclonal antibodies;
D O I
10.1016/S0264-410X(02)00133-0
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
An ELISA-immunocapture assay was developed for quantification of influenza neuraminidases (N2 and B), with a sensitivity about 10 ng neuramidase (NA)/ml. This sensitive and specific assay was applied to several trivalent vaccine preparations (1997/1998 and 1998/1999). It was shown capable to detect 3.5-6.9 mug NA/ml according to the influenza strain and the vaccine origin. (C) 2002 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:S59 / S60
页数:2
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