RETRACTED: Foxm1 Mediates LIF/Stat3-Dependent Self-Renewal in Mouse Embryonic Stem Cells and Is Essential for the Generation of Induced Pluripotent Stem Cells (Retracted Article)

被引:19
作者
Tan, Guixiang [1 ]
Cheng, Liang [1 ]
Chen, Tuanhui [1 ]
Yu, Li [1 ]
Tan, Yongjun [1 ]
机构
[1] Hunan Univ, State Key Lab Chemo Biosensing & Chemometr, Dept Biomed Engn, Coll Biol,Collaborat Innovat Ctr Chem & Mol Med, Changsha 410082, Hunan, Peoples R China
来源
PLOS ONE | 2014年 / 9卷 / 04期
关键词
LEUKEMIA INHIBITORY FACTOR; TRANSCRIPTION FACTOR; SIGNALING PATHWAYS; CARCINOMA-CELLS; BREAST-CANCER; GROUND-STATE; TUMOR-GROWTH; EXPRESSION; ACTIVATION; PROMOTES;
D O I
10.1371/journal.pone.0092304
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Activation of signal transducer and activator of transcription 3 (Stat3) by leukemia inhibitory factor (LIF) is required for maintaining self-renewal and pluripotency of mouse embryonic stem cells (mESCs). Here, we have confirmed transcription factor Forkhead Box m1 (Foxm1) as a LIF/Stat3 downstream target that mediates LIF/Stat3-dependent mESC self-renewal. The expression of Foxm1 relies on LIF signaling and is stimulated by Stat3 directly in mESCs. The knockdown of Foxm1 results in the loss of mESC pluripotency in the presence of LIF, and the overexpression of Foxm1 alone maintains mESC pluripotency in the absence of LIF and feeder layers, indicating that Foxm1 is a mediator of LIF/Stat3-dependent maintenance of pluripotency in mESCs. Furthermore, the inhibition of Foxm1 expression prevents the reprogramming of mouse embryonic fibroblasts to induced pluripotent stem cells (iPSCs), suggesting that Foxm1 is essential for the reprogramming of somatic cells into iPSCs. Our results reveal an essential function of Foxm1 in the LIF/Stat3-mediated mESC self-renewal and the generation of iPSCs.
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页数:10
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