Stability, purification, and applications of bromelain: A review

被引：107

作者：

de Lencastre Novaes, Leticia Celia ^{[1
,2
]}

Jozala, Angela Faustino ^{[3
]}

Lopes, Andre Moreni ^{[1
]}

Santos-Ebinuma, Valeria de Carvalho ^{[4
]}

Mazzola, Priscila Gava ^{[2
]}

Pessoa Junior, Adalberto ^{[2
]}

机构：

[1] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Biochem & Pharmaceut Technol, Sao Paulo, Brazil

[2] Univ Estadual Campinas, UNICAMP, Fac Pharmaceut Sci, Campinas, SP, Brazil

[3] Sorocaba Univ, Sch Pharmaceut Sci, Dept Pharm, Sorocaba, Brazil

[4] UNESP Univ Estadual Paulista, Sch Pharmaceut Sci, Dept Bioproc & Biotechnol, Araraquara, Brazil

来源：

BIOTECHNOLOGY PROGRESS | 2016年 / 32卷 / 01期

关键词：

bromelain; protease; anti-inflammatory; downstream processes; pineapple; purification; LIQUID-LIQUID-EXTRACTION; PINEAPPLE ANANAS-COMOSUS; STEM BROMELAIN; IN-VITRO; ENZYMATIC DEBRIDEMENT; PROTEOLYTIC-ENZYME; EC; 3.4.22.33; SEPARATION; TRANSPORT; PAPAIN;

D O I：

10.1002/btpr.2190

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Bromelain is a cysteine protease found in pineapple tissue. Because of its anti-inflammatory and anti-cancer activities, as well as its ability to induce apoptotic cell death, bromelain has proved useful in several therapeutic areas. The market for this protease is growing, and several studies exploring various properties of this molecule have been reported. This review aims to compile this data, and summarize the main findings on bromelain in the literature to date. The physicochemical properties and stability of bromelain under different conditions are discussed. Several studies on the purification of bromelain from crude extracts using a wide range of techniques such as liquid-liquid extractions by aqueous two-phase system, ultrafiltration, precipitation, and chromatography, have been reported. Finally, the various applications of bromelain are presented. This review therefore covers the main properties of bromelain, aiming to provide an up-to-date compilation of the data reported on this enzyme. (c) 2015 American Institute of Chemical Engineers Biotechnol. Prog., 32:5-13, 2016

引用

页码：5 / 13

页数：9

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